Blackman R L, Malarky G, Margaritopoulos J T, Tsitsipis J A
Department of Entomology, The Natural History Museum, London, SW7 5BD, UK.
Bull Entomol Res. 2007 Jun;97(3):253-63. doi: 10.1017/S0007485307004907.
Microsatellite genotyping was used to identify common clones in populations of the Myzus persicae group from various hosts and regions in mainland Greece and southern Italy and to compare their distribution and occurrence on tobacco and other crops. Common clones were defined as genotypes collected at more than one time or in more than one population; and, therefore, unlikely to be participating in the annual sexual phase on peach. Sixteen common genotypes were found, accounting for 49.0% of the 482 clonal lineages examined. Eight of these genotypes were subjected, in the laboratory, to short days and found to continue parthenogenetic reproduction, i.e. they were anholocyclic. Four of the six commonest genotypes were red, and one of these accounted for 29.6% of the samples from tobacco and 29.4% of those from overwintering populations on weeds. All six commonest genotypes were found on weeds and five of them both on tobacco and on other field crops. In mainland Greece, the distribution of common clones corresponded closely with that of anholocyclic lineages reported in a previous study of life cycle variation. Common genotypes were in the minority in the commercial peach-growing areas in the north, except on weeds in winter and in tobacco seedbeds in early spring, but predominated further south, away from peach trees. This contrasts with the situation in southern Italy, reported in a previous paper, where peaches were available for the sexual phase, yet all samples from tobacco were of common genotypes.
微卫星基因分型被用于识别来自希腊大陆和意大利南部不同寄主及地区的烟蚜种群中的常见克隆，并比较它们在烟草和其他作物上的分布及出现情况。常见克隆被定义为在多个时间点或多个种群中采集到的基因型；因此，它们不太可能参与桃树上的年度有性阶段。共发现了16种常见基因型，占所检测的482个克隆谱系的49.0%。在实验室中，对其中8种基因型进行短日照处理，发现它们能继续孤雌生殖，即它们是无全周期的。最常见的6种基因型中有4种是红色的，其中一种占烟草样本的29.6%，占杂草上越冬种群样本的29.4%。在杂草上发现了所有6种最常见的基因型，其中5种在烟草和其他大田作物上也有发现。在希腊大陆，常见克隆的分布与先前关于生命周期变异的研究中报道的无全周期谱系的分布密切相关。在北部的商业桃种植区，常见基因型占少数，除了冬季杂草上和早春烟草苗床上的基因型，但在更南部远离桃树的地区占主导地位。这与先前一篇论文中报道的意大利南部的情况形成对比，在那里桃树可用于有性阶段，但来自烟草的所有样本都是常见基因型。
