Margaritopoulos J T, Gondosopoulos B, Mamuris Z, Skouras P J, Voudouris K C, Bacandritsos N, Fantinou A A, Tsitsipis J A
Laboratory of Entomology and Agricultural Zoology, Department of Agriculture, Crop Production and Rural Environment, University of Thessaly, Fytokou Str., 38446 Nea Ionia, Magnesia, Greece.
Bull Entomol Res. 2007 Jun;97(3):299-308. doi: 10.1017/S000748530700507X.
Restriction fragment length polymorphism analysis of two segments of mitochondrial DNA (COI and 16S rRNA) was used to examine genetic variation in Sesamia nonagrioides (Lefèbvre) populations from the Mediterranean basin. Four populations were collected from central and southern Greece, and five from northern latitudes: Greece, Italy, France and Spain. No variation was observed in COI, while 16S rRNA segment proved highly polymorphic and 28 different haplotypes were found. Lower intra-population polymorphism was found in the northern populations than in southern ones. Although no significant isolation by distance was found, the UPGMA tree based on Nei's raw number of nucleotide differences separated the populations into two major groups, i.e. one with the northern (40.6 degrees N-43.4 degrees N) and the other with the southern populations (37.3 degrees N-39.2 degrees N). Analysis of molecular variance revealed that most of the variation was between the two major groups (Phi(CT)=0.559), and all pairwise comparisons between the northern and southern populations resulted in high and significant F(ST) values (overall F(ST)=0.604). The high F(ST) values and the strong spatial genetic structure indicate that long-distance migration may be a rare event. The populations do not seem to have experienced a strong historical bottleneck. The occurrence of a few widespread haplotypes and the genetic similarity of the northern populations could be attributed to a historical expansion of certain haplotypes from the south towards to the northern borders of the species' distribution area.
利用线粒体DNA的两个片段（细胞色素氧化酶亚基I基因（COI）和16S核糖体RNA（16S rRNA））的限制性片段长度多态性分析，来检测地中海盆地地区的非裔蔗螟（Sesamia nonagrioides (Lefèbvre)）种群的遗传变异。从希腊中部和南部采集了4个种群，从北纬地区（希腊、意大利、法国和西班牙）采集了5个种群。在COI基因中未观察到变异，但16S rRNA片段显示出高度多态性，共发现了28种不同的单倍型。北部种群的种群内多态性低于南部种群。虽然未发现显著的距离隔离现象，但基于Nei氏核苷酸差异原始数量构建的UPGMA树将这些种群分为两个主要类群，即一个类群包含北部种群（北纬40.6度至43.4度），另一个类群包含南部种群（北纬37.3度至39.2度）。分子方差分析表明，大部分变异存在于两个主要类群之间（Phi(CT)=0.559），并且北部和南部种群之间的所有成对比较都产生了高且显著的F(ST)值（总体F(ST)=0.604）。高F(ST)值和强烈的空间遗传结构表明远距离迁移可能是罕见事件。这些种群似乎没有经历过强烈的历史瓶颈。少数广泛分布的单倍型的出现以及北部种群的遗传相似性可能归因于某些单倍型从南部向该物种分布区域北部边界的历史扩张。
