Yue Hai-ying, Cao Ji, Cui Ji-feng, Dai Zhi, Su Jian-jia, Duan Xiao-xian, Yang Chun, Yue Hui-fen, Li Yuan, Liu Yin-kun
Affiliated Tumor Hospital of Guangxi Medical University, Nanning 530021, China.
Zhonghua Gan Zang Bing Za Zhi. 2007 May;15(5):366-9.
To evaluate the mRNA and protein expressions of peroxiredoxin II (PrxII) in hepatocellular carcinoma (HCC) and their significance.
HCC was induced by aflatoxin B1 (AFB1) in 6 tree shrews (Tupaia belangeri chinensis). The expression levels of PrxII mRNA and protein were detected by reverse transcriptase polymerase chain reaction (RT-PCR) and Western blot on HCC tissues and on their surrounding liver tissues (para-HCC). Biopsied liver tissues were taken before the HCC induction (pre-HCC) from the same animals and from a group of blank controlled animals that served as controls. Liver biopsy specimens from 18 cases of human HCC and from 17 healthy human volunteers were studied using the same methods.
The mRNA and protein expressions of PrxII in tree shrew HCC tissues were significantly higher than those in para-HCC and pre-HCC tissues, and also higher than those in the liver tissues from the control animals (all P < 0.05). The expression levels of PrxII mRNA and protein in human HCC tissues were also significantly higher than those in their para-HCC tissues and in the human normal liver tissues (P < 0.05).
PrxII might play an important role in hepatocarcinogenesis and might be used as a molecular target for HCC prevention and treatment.
评估过氧化物酶Ⅱ（PrxⅡ）在肝细胞癌（HCC）中的mRNA和蛋白表达及其意义。
用黄曲霉毒素B1（AFB1）诱导6只树鼩（中缅树鼩）发生HCC。采用逆转录聚合酶链反应（RT-PCR）和蛋白质印迹法检测HCC组织及其周围肝组织（癌旁肝组织）中PrxⅡ mRNA和蛋白的表达水平。从同一组动物以及作为对照的空白对照组动物中，在诱导HCC之前（癌前肝组织）获取肝脏活检组织。采用相同方法研究18例人类HCC患者的肝脏活检标本以及17名健康人类志愿者的肝脏活检标本。
树鼩HCC组织中PrxⅡ的mRNA和蛋白表达显著高于癌旁肝组织和癌前肝组织，也高于对照动物的肝组织（均P<0.05）。人类HCC组织中PrxⅡ mRNA和蛋白的表达水平也显著高于其癌旁肝组织和正常肝组织（P<0.05）。
PrxⅡ可能在肝癌发生过程中起重要作用，有望作为HCC防治的分子靶点。
