Matsuoka Keisuke, Hirosawa Takashi, Honda Chikako, Endo Kazutoyo, Moroi Yoshikiyo, Shibata Osamu
Department of Physical Chemistry, Showa Pharmaceutical University, Higashi-Tamagawagakuen 3-3165, Machida, Tokyo, Japan.
Chem Phys Lipids. 2007 Jul;148(1):51-60. doi: 10.1016/j.chemphyslip.2007.04.007. Epub 2007 Apr 21.
Differences in the preferential solubilization of cholesterol and competitive solubilizates (beta-sitosterol and aromatic compounds) in bile salt micelles was systematically studied by changing the molar ratio of cholesterol to competitive solubilizates. The cholesterol solubility in a mixed binary system (cholesterol and beta-sitosterol) was almost half that of the cholesterol alone system, regardless of the excess beta-sitosterol quantity added. On the other hand, the mutual solubilities of cholesterol and pyrene were not inhibited by their presence in binary mixed crystals. Finally, the cholesterol solubility was measured by changing the alkyl chain length of n-alkylbenzenes. When tetradecylbenzene was added to the bile solution, the cholesterol solubility decreased slightly and was below the original cholesterol solubility. Based on Gibbs energy change (DeltaG degrees ) for solubilization, chemicals that inhibit cholesterol solubility in their combined crystal systems showed a larger negative DeltaG degrees value than cholesterol alone.
通过改变胆固醇与竞争性增溶剂（β-谷甾醇和芳香族化合物）的摩尔比，系统研究了胆汁盐胶束中胆固醇和竞争性增溶剂的优先增溶差异。在二元混合体系（胆固醇和β-谷甾醇）中，胆固醇的溶解度几乎是单独胆固醇体系的一半，无论添加的β-谷甾醇过量多少。另一方面，胆固醇和芘在二元混合晶体中的相互溶解度不受其存在的抑制。最后，通过改变正烷基苯的烷基链长度来测量胆固醇的溶解度。当向胆汁溶液中加入十四烷基苯时，胆固醇的溶解度略有下降，且低于原始胆固醇溶解度。基于增溶的吉布斯自由能变化（ΔG°），在其组合晶体体系中抑制胆固醇溶解度的化学物质显示出比单独胆固醇更大的负ΔG°值。
