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辅助生殖技术实验室中的培养箱管理

Incubator management in an assisted reproductive technology laboratory.

作者信息

Higdon H Lee, Blackhurst Dawn W, Boone William R

机构信息

Department of Obstetrics and Gynecology, Greenville Hospital System University Medical Group, Greenville, SC 29605-5601, USA.

出版信息

Fertil Steril. 2008 Mar;89(3):703-10. doi: 10.1016/j.fertnstert.2007.03.040. Epub 2007 May 24.

Abstract

OBJECTIVE

To study the effect of incubator management on assisted reproductive technology (ART) outcomes.

DESIGN

Series of retrospective and controlled, randomized studies.

SETTING

Tertiary care infertility practice.

PATIENT(S): Mammalian gametes/embryos.

INTERVENTION(S): Evaluation of human and bovine oocytes/embryos cultured in various environmental conditions.

MAIN OUTCOME MEASURE(S): Fertilization and embryo development rate as well as clinical pregnancy rate (PR).

CONCLUSION(S): Here we review the general topic of incubator management as it pertains to ART. Discussed within the context of this article will be our experiences as they relate to incubator management. Details as they apply to incubator environment also will include gamete/embryo positions within incubator, air quality, and quality control.

摘要

目的

研究培养箱管理对辅助生殖技术(ART)结局的影响。

设计

一系列回顾性、对照、随机研究。

地点

三级医疗不孕症诊疗机构。

研究对象

哺乳动物配子/胚胎。

干预措施

评估在各种环境条件下培养的人及牛的卵母细胞/胚胎。

主要观察指标

受精率、胚胎发育率以及临床妊娠率(PR)。

结论

在此,我们综述与ART相关的培养箱管理这一总体主题。本文将讨论我们在培养箱管理方面的经验。适用于培养箱环境的细节还将包括配子/胚胎在培养箱内的位置、空气质量和质量控制。

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