Wrenzycki C, Herrmann D, Niemann H
Institute for Animal Breeding (FAL), Department of Biotechnology, Mariensee, 31535 Neustadt, Germany.
Theriogenology. 2007 Sep 1;68 Suppl 1:S77-83. doi: 10.1016/j.theriogenology.2007.04.028. Epub 2007 May 23.
Messenger RNA (mRNA) expression techniques have become a powerful tool to analyze the relative abundance of transcripts related to oocyte and/or embryo quality. Numerous efforts to identify candidate genes for the developmental competence of bovine oocytes and embryos have been made employing different strategies. The preimplantation bovine embryo is initially under the control of maternal genomic information that is accumulated during oogenesis. Soon, the genetic program of development becomes dependent upon new transcripts derived from activation of the embryonic genome. The early steps in development including maturation, fertilization, timing of first cleavage, activation of the embryonic genome, compaction, and blastocyst formation can be affected by the culture media and conditions as well as the production procedure itself. These perturbations can possibly result in a dramatic decrease of the quality of the resulting blastocysts, and may even affect the viability of offspring born after transfer.
信使核糖核酸（mRNA）表达技术已成为分析与卵母细胞和/或胚胎质量相关转录本相对丰度的有力工具。人们采用不同策略，为确定牛卵母细胞和胚胎发育能力的候选基因付出了诸多努力。植入前的牛胚胎最初受卵母细胞发生过程中积累的母体基因组信息控制。很快，发育的遗传程序就依赖于胚胎基因组激活产生的新转录本。发育的早期步骤，包括成熟、受精、首次卵裂时间、胚胎基因组激活、致密化和囊胚形成，会受到培养基和培养条件以及生产过程本身的影响。这些干扰可能导致所产生囊胚质量急剧下降，甚至可能影响移植后出生后代的活力。
