通过DNA生物传感器评估碳酸介质中铂（II）抗增殖药物的活化作用。

The activation of platinum(II) antiproliferative drugs in carbonate medium evaluated by means of a DNA-biosensor.

作者信息

Ravera Mauro, Bagni Graziana, Mascini Marco, Dabrowiak James C, Osella Domenico

机构信息

Dipartimento di Scienze dell'Ambiente e della Vita, Università del Piemonte Orientale, Via Bellini 25g, 15100 Alessandria, Italy.

出版信息

J Inorg Biochem. 2007 Jul;101(7):1023-7. doi: 10.1016/j.jinorgbio.2007.03.017. Epub 2007 Apr 19.

DOI:10.1016/j.jinorgbio.2007.03.017

PMID:17524484

Abstract

We report on the binding of cisplatin, carboplatin and oxaliplatin to double-stranded DNA in two different (phosphate and carbonate) buffers, using an electrochemical DNA-biosensor. The propensity of the electrophilic agent produced by hydrolysis to interact with DNA was measured as a function of the decrease of guanine oxidation signal of the metal-DNA adduct immobilized on a screen-printed electrode, by using square wave voltammetry. The results obtained confirm that carbonate reacts with platinum drugs to form activated carbonato complexes, which are able to react readily with DNA.

摘要

我们使用电化学DNA生物传感器，报告了顺铂、卡铂和奥沙利铂在两种不同（磷酸盐和碳酸盐）缓冲液中与双链DNA的结合情况。通过方波伏安法，测量水解产生的亲电试剂与DNA相互作用的倾向，该倾向是固定在丝网印刷电极上的金属-DNA加合物鸟嘌呤氧化信号降低的函数。获得的结果证实，碳酸盐与铂类药物反应形成活性碳酸根络合物，这些络合物能够很容易地与DNA反应。