Ciarlo Monica, Bruzzone Federica, Angelini Cristiano, Vallarino Mauro, Vaudry Hubert
Department of Biology, University of Genova, 16132 Genova, Italy.
Peptides. 2007 Sep;28(9):1738-45. doi: 10.1016/j.peptides.2007.04.010. Epub 2007 Apr 22.
The distribution of pituitary adenylate cyclase-activating polypeptide (PACAP) and PACAP receptors in the brain of amphibians has been previously described. In the present study, we have investigated the ontogeny of the selective PACAP receptor, PAC1-R, and the PACAP-vasoactive intestinal polypeptide (VIP) mutual receptor, VPAC1-R, in frog embryos by whole-mount in situ hybridization histochemistry. At stage 20, expression of PAC1-R and/or VPAC1-R mRNAs was detected in the brain, the auditory vesicles, the external gills, the buds of the lateral lines and the coelomatic cavity. At stage 25, PAC1-R and/or VPAC1-R mRNAs were observed in the buds of the orbital lateral line, the pancreas and heart. At stage 30, PAC1-R and VPAC1-R mRNAs were widely distributed in the telencephalon and diencephalon as well as in the bud of the lateral line, the heart and the pancreas. The anatomical distribution of PAC1-R and VPAC1-R mRNAs, although similar, did not totally overlap, indicating that PACAP and VIP may exert differential effects in frog during development.
垂体腺苷酸环化酶激活多肽（PACAP）及其受体在两栖动物大脑中的分布已有相关描述。在本研究中，我们通过整装原位杂交组织化学方法，研究了蛙胚中选择性PACAP受体PAC1-R和PACAP-血管活性肠肽（VIP）共同受体VPAC1-R的个体发生。在第20阶段，在大脑、听泡、外鳃、侧线芽和体腔中检测到PAC1-R和/或VPAC1-R mRNA的表达。在第25阶段，在眶侧线芽、胰腺和心脏中观察到PAC1-R和/或VPAC1-R mRNA。在第30阶段，PAC1-R和VPAC1-R mRNA广泛分布于端脑和间脑，以及侧线芽、心脏和胰腺。PAC1-R和VPAC1-R mRNA的解剖分布虽然相似，但并不完全重叠，这表明PACAP和VIP在蛙发育过程中可能发挥不同的作用。
