Zabala A, Portillo M P, Navarro V, Macarulla M T, Barron L J R, Fernández-Quintela A
Department of Nutrition and Food Science, University of the Basque Country, Paseo de la Universidad 7, Vitoria, Spain.
J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Aug 15;855(2):152-8. doi: 10.1016/j.jchromb.2007.04.041. Epub 2007 May 10.
A quantitative GC method for conjugated linoleic acid (CLA) isomers of physiological significance (cis-9, trans-11 CLA and trans-10, cis-12 CLA) as non-esterified fatty acids (NEFA) or triacylglycerols (TAG) was developed. Furthermore, the effect of the internal standard addition point (sample or fat extract) was studied. Response linearity, recovery and precision assays, detection and quantification limits were determined. Linearity was demonstrated over a range from 0.1 to 10 microg/mL. When CLA isomers were present as NEFA, the recovery significantly decreased (P< or =0.05) from 76% to 27.1% (cis-9, trans-11 CLA) and 28.5% (trans-10, cis-12 CLA) when the standards were added to the fat extract or to the initial tissue, respectively. As an application, liver samples from hamsters fed a diet supplemented with both CLA isomers were analyzed. The CLA isomers in liver samples were detected with reasonable reproducibility.
建立了一种定量气相色谱法，用于分析具有生理意义的共轭亚油酸（CLA）异构体（顺式-9，反式-11 CLA和反式-10，顺式-12 CLA），其以非酯化脂肪酸（NEFA）或三酰甘油（TAG）形式存在。此外，还研究了内标添加点（样品或脂肪提取物）的影响。测定了响应线性、回收率和精密度，以及检测限和定量限。在0.1至10μg/mL范围内表现出线性。当CLA异构体以NEFA形式存在时，分别将标准品添加到脂肪提取物或初始组织中时，回收率显著降低（P≤0.05），顺式-9，反式-11 CLA从76%降至27.1%，反式-10，顺式-12 CLA从76%降至28.5%。作为应用，分析了喂食添加两种CLA异构体日粮的仓鼠的肝脏样本。肝脏样本中的CLA异构体检测具有合理的重现性。
