Curtis Rosane H C
Nematode Interactions Unit, Division of Plant-Pathogen Interactions, Rothamsted-Research, Harpenden, Hertfordshire, UK.
Brief Funct Genomic Proteomic. 2007 Mar;6(1):50-8. doi: 10.1093/bfgp/elm006. Epub 2007 May 24.
This review focuses on the proteins and secretions of sedentary plant parasitic nematodes potentially important for plant-nematode interactions. These nematodes are well equipped for parasitism of plants. Having acquired the ability to manipulate fundamental aspects of plant biology, they are able to hijack host-cell development to make their feeding site. They feed exclusively from feeding sites as they complete their life cycle, satisfying their nutritional demands for development and reproduction. Biochemical and genomic approaches have been used successfully to identify a number of nematode parasitism genes. So far, 65 204 expressed sequence tags (ESTs) have been generated for six Meloidogyne species and sequencing projects, currently in progress, will underpin genomic comparisons of Meloidogyne spp. with sequences of other pathogens and generate genechip microarrays to undertake profiling studies of up- and down-regulated genes during the infection process. RNA interference provides a molecular genetic tool to study gene function in parasitism. These methods should provide new data to help our understanding of how parasitic nematodes infect their hosts, leading to the identification of novel pathogenicity genes.
本综述聚焦于久坐型植物寄生线虫的蛋白质和分泌物，这些对于植物与线虫的相互作用可能具有重要意义。这些线虫具备良好的植物寄生能力。它们获得了操纵植物生物学基本方面的能力，能够劫持宿主细胞发育以形成其取食位点。在完成生命周期的过程中，它们仅从取食位点获取营养，满足其发育和繁殖的营养需求。生化和基因组学方法已成功用于鉴定一些线虫寄生基因。到目前为止，已针对六种根结线虫物种生成了65204个表达序列标签（EST），目前正在进行的测序项目将为根结线虫属与其他病原体序列的基因组比较提供支持，并生成基因芯片微阵列以进行感染过程中上调和下调基因的谱分析研究。RNA干扰提供了一种分子遗传学工具来研究寄生中的基因功能。这些方法应能提供新的数据，以帮助我们理解寄生线虫如何感染其宿主，从而鉴定出新的致病基因。
