Joyce Margaret M, Burghardt Robert C, Geisert Rodney D, Burghardt James R, Hooper R Neil, Ross Jason W, Ashworth Morgan D, Johnson Greg A
Center for Animal Biotechnology and Genomics, Department of Veterinary Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, Texas 77843, USA.
Endocrinology. 2007 Sep;148(9):4420-31. doi: 10.1210/en.2007-0505. Epub 2007 May 24.
Conceptus trophectoderm and uterine luminal epithelial cells interact via endocrine, paracrine, and autocrine modulators to mediate pregnancy recognition and implantation. Pig conceptuses not only release estrogens for pregnancy recognition but also secrete interferons during implantation. Because interferon-stimulated genes are increased by interferons secreted for pregnancy recognition in ruminants, we asked whether the interferon-stimulated gene, STAT1, is up-regulated in pig endometrium by conceptus estrogens and/or interferons. STAT1 expression in response to day of pregnancy, estrogen injection, and intrauterine infusion of conceptus secretory proteins in pigs indicated 1) estrogen increases STAT1 in luminal epithelial cells, 2) conceptus secretory proteins that contain interferons increase STAT1 in stroma, 3) STAT1 increases in close proximity to the conceptus, and 4) early estrogen results in conceptus death and no STAT1 in stroma. The interactions of estrogen and interferons to regulate cell-type-specific expression of STAT1 highlight the complex interplay between endometrium and conceptus for pregnancy recognition and implantation.
胚胎滋养外胚层与子宫腔上皮细胞通过内分泌、旁分泌和自分泌调节因子相互作用，以介导妊娠识别和着床。猪胚胎不仅释放雌激素用于妊娠识别，在着床期间还分泌干扰素。由于在反刍动物中，妊娠识别所分泌的干扰素会增加干扰素刺激基因，我们研究了干扰素刺激基因STAT1在猪子宫内膜中是否会因胚胎雌激素和/或干扰素而上调。猪中STAT1的表达对妊娠天数、雌激素注射以及子宫内注入胚胎分泌蛋白的反应表明：1）雌激素增加腔上皮细胞中的STAT1；2）含有干扰素的胚胎分泌蛋白增加基质中的STAT1；3）STAT1在胚胎附近增加；4）早期雌激素导致胚胎死亡且基质中无STAT1。雌激素和干扰素相互作用调节STAT1的细胞类型特异性表达，突出了子宫内膜与胚胎在妊娠识别和着床过程中的复杂相互作用。
