Diogenes A, Akopian A N, Hargreaves K M
Departments of Pharmacology, University of Texas Health Science Center, 7703 Floyd Curl Drive, San Antonio, TX 78229-3900, USA.
J Dent Res. 2007 Jun;86(6):550-5. doi: 10.1177/154405910708600612.
The transient receptor potential ankyrin repeat 1 (TRPA1) channel is believed to be involved in many forms of acute and chronic hyperalgesia. Nerve Growth Factor (NGF) regulates chronic inflammatory hyperalgesia by controlling gene expression in sensory neurons, including genes involved in inflammatory hyperalgesia in the dental pulp. We hypothesized that NGF increases functional activities of the TRPA1 channel in trigeminal ganglion neurons. Here, we show that NGF induced a concentration- and time-dependent up-regulation of TRPA1 mRNA in trigeminal ganglia neurons, as detected by real-time RT-PCR and in situ hybridization. In addition, NGF evoked a time-dependent increase of mustard oil (MO)-evoked TRPA1 activation in trigeminal ganglia neurons. Collectively, these findings demonstrate that NGF participates in the functional up-regulation of TRPA1 in trigeminal ganglia neurons. These enhanced activities of TRPA1 could play an important role in the development of hyperalgesia following nerve injury and inflammation in the orofacial region.
瞬时受体电位锚蛋白重复序列1（TRPA1）通道被认为与多种形式的急性和慢性痛觉过敏有关。神经生长因子（NGF）通过控制感觉神经元中的基因表达来调节慢性炎症性痛觉过敏，这些基因包括参与牙髓炎症性痛觉过敏的基因。我们推测NGF会增加三叉神经节神经元中TRPA1通道的功能活性。在此，我们发现，通过实时逆转录-聚合酶链反应（RT-PCR）和原位杂交检测，NGF在三叉神经节神经元中诱导了TRPA1 mRNA浓度和时间依赖性的上调。此外，NGF在三叉神经节神经元中引起了芥子油（MO）诱发的TRPA1激活的时间依赖性增加。总的来说，这些发现表明NGF参与了三叉神经节神经元中TRPA1的功能上调。TRPA1这些增强的活性可能在口面部区域神经损伤和炎症后痛觉过敏的发展中起重要作用。
