磷酸化核仁非组蛋白的DNA-纤维素柱层析
DNA-cellulose column chromatography of phosphorylated nucleolar nonhistone proteins.
作者信息
Bearden J, Chandra T
出版信息
Mol Cell Biochem. 1976 Jan 31;10(1):3-9. doi: 10.1007/BF01731675.
PMID:175259
Abstract
A salt-extraction procedure was used to isolate a nucleolar nonhistone protein fraction, containing [32P]phosphoserine, from the nucleoli of Novikoff hepatoma ascites cells. These proteins are similar in amino-acid composition to whole nuclear (chromosomal) nonhistone proteins. DNA-cellulose column chromatography showed that this fraction contains DNA-binding phosphoproteins, some of which will bind only to homologous (Novikoff) nucleolar or nuclear DNA.
摘要
采用盐提取法从诺维科夫肝癌腹水细胞的核仁中分离出一种含有[32P]磷酸丝氨酸的核仁非组蛋白部分。这些蛋白质在氨基酸组成上与整个细胞核（染色体）非组蛋白相似。DNA-纤维素柱层析表明，该部分含有与DNA结合的磷蛋白，其中一些仅与同源（诺维科夫）核仁或核DNA结合。
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