Gao Yue-qiu, Zheng Ya-jiang, Wang Ling-tai
Affiliated Shuguang Hospital, Shanghai University of TCM, Shanghai.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2007 Apr;27(4):300-2.
To observe the effect of Spleen-invigorating Prescription (SIP) on dendritic cell function in patients with chronic hepatitis B.
A total of 60 patients with chronic HBV of Pi-deficiency syndrome type were enrolled and randomized to 2 groups, 30 in each group. Patients in the control group were given intramuscular injection with human interferon alpha 1b, 3 times a week, while those in the treated group were given orally with SIP twice a day, the therapy lasted for 6 months. Dendritic cells (DCs) were isolated from peripheral blood and cultured, then the expression of surface markers, HLA-DR, CD86, CD80, CD40, CD14 and CD11c were detected before and after treatment by flow cytometry, and the function of DCs was also evaluated by mixed lymphocyte reaction (MLR) determination once before treatment and once after treatment.
The expressions of DCs' surface CD86, CD80, CD40 and CD11c in the treated group were higher (P < 0.05, P < 0.01) and the changes of stimulating index, IFN-gamma and IL-12 were superior in the treated group to those in the control group (P < 0.05).
SIP can significantly improve DC's function, so, one of mechanisms of SIP in improving clinical efficacy may be the regulation of immune function.
观察健脾方对慢性乙型肝炎患者树突状细胞功能的影响。
选取60例慢性乙型肝炎脾虚证患者，随机分为2组，每组30例。对照组患者每周3次肌肉注射人α1b干扰素，治疗组患者每日2次口服健脾方，疗程均为6个月。分离外周血树突状细胞（DC）进行培养，采用流式细胞术检测治疗前后DC表面标志物HLA-DR、CD86、CD80、CD40、CD14及CD11c的表达，并于治疗前后各进行1次混合淋巴细胞反应（MLR）检测以评估DC功能。
治疗组DC表面CD86、CD80、CD40及CD11c的表达较高（P<0.05，P<0.01），且治疗组刺激指数、IFN-γ及IL-12的变化优于对照组（P<0.05）。
健脾方可显著改善DC功能，因此，健脾方提高临床疗效的机制之一可能是调节免疫功能。
