Varloud M, Fonty G, Roussel A, Guyonvarch A, Julliand V
EVIALIS, 56250 Saint-Nolff, France.
J Anim Sci. 2007 Oct;85(10):2508-16. doi: 10.2527/jas.2006-182. Epub 2007 May 25.
Our knowledge of the microflora of the stomach of the horse is still limited, although some data indicate its important role in nutrition. The objective of this experiment was to investigate the microbial and biochemical profiles in the stomach of the horse and to quantify the disappearance of dietary starch. Total anaerobic bacteria, lactate-utilizing bacteria, lactobacilli, and streptococci were determined, and biochemical characteristics (pH, and DM, D- and L-lactate, D-glucose, NH3, and VFA concentrations) were measured in chyme collected from 4 horses by naso-gastric intubation aided by endoscopy, at 30 min before and 60, 120, and 210 min after the meal. The total anaerobic population exhibited a linear increase (5.54 to 6.98 log10 cfu/mL; P = 0.018) within the first postprandial hour and reached 8.32 log10 cfu/mL at 210 min after the meal. The concentrations of lactobacilli, streptococci, and lactate-utilizing bacteria in the stomach contents were 5.52, 4.82, and 6.95 log10 cfu/mL, respectively. Lactate concentration increased linearly from 0.25 mmol/L before the meal to 7.98 mmol/L at the last collection point (P = 0.013). This increase was mostly due to L-lactate accumulation. The VFA concentration increased linearly (P = 0.002) during the postprandial period from 1.96 to 8.17 mmol/L. Acetate represented, on average, 78 mol/100 mol of total VFA. The average concentration of NH3 in the stomach content was 2.48 mmol/L. Dietary starch disappearance did not respond during the post-prandial period and was not consistent with previous findings. These in vivo data provide complementary information on the postprandial microbial and biochemical kinetics in the stomachs of horses and confirm its abundant microbial colonization.
尽管一些数据表明马胃微生物群在营养方面具有重要作用，但我们对其的了解仍然有限。本实验的目的是研究马胃中的微生物和生化特征，并量化日粮淀粉的消失情况。通过在内镜辅助下经鼻胃插管，从4匹马收集食糜，测定了总厌氧菌、利用乳酸的细菌、乳酸杆菌和链球菌，并测量了生化特性（pH值、干物质、D-和L-乳酸、D-葡萄糖、NH₃和挥发性脂肪酸浓度），分别在进食前30分钟以及进食后60、120和210分钟进行。总厌氧菌数量在餐后第一小时内呈线性增加（从5.54增至6.98 log₁₀ cfu/mL；P = 0.018），并在进食后210分钟达到8.32 log₁₀ cfu/mL。胃内容物中乳酸杆菌、链球菌和利用乳酸的细菌浓度分别为5.52、4.82和6.95 log₁₀ cfu/mL。乳酸浓度从进食前的0.25 mmol/L线性增加到最后一个采集点的7.98 mmol/L（P = 0.013）。这种增加主要是由于L-乳酸的积累。餐后期间挥发性脂肪酸浓度从1.96 mmol/L线性增加到8.17 mmol/L（P = 0.002）。乙酸平均占总挥发性脂肪酸的78 mol/100 mol。胃内容物中NH₃的平均浓度为2.48 mmol/L。餐后期间日粮淀粉的消失情况没有反应，与先前的研究结果不一致。这些体内数据提供了关于马胃餐后微生物和生化动力学的补充信息，并证实了其丰富的微生物定植情况。
