Nagaleekar V K, Tiwari A K, Kataria R S, Bais M V, Ravindra P V, Kumar S
Given D305, Burlington, VT, USA.
Arch Virol. 2007;152(9):1751-6. doi: 10.1007/s00705-007-0988-z. Epub 2007 May 26.
Bluetongue virus (BTV) causes haemorrhagic disease in sheep and induces death in cultured mammalian cells. In the present study, BTV-induced apoptotic pathways in Vero cells were elucidated. Cells infected with BTV at 0.1 m.o.i underwent DNA fragmentation and membrane blebbing within 48 h postinfection. BTV-induced apoptosis was blocked by the pan-caspase inhibitor, z-VAD-FMK. Immuno-blotting using anti-caspase-8 and -9 antibodies detected the activation of the respective caspases. Flow cytometry analyses following 3, 3' dihexyloxacarbocyanine iodide staining revealed the loss of mitochondrial membrane potential. Our study confirms the involvement of both caspase-dependent extrinsic and intrinsic pathways of apoptosis in BTV-infected cells.
蓝舌病毒（BTV）可导致绵羊出血性疾病，并在培养的哺乳动物细胞中引发死亡。在本研究中，阐明了BTV在Vero细胞中诱导的凋亡途径。以0.1感染复数感染BTV的细胞在感染后48小时内发生DNA片段化和细胞膜起泡。BTV诱导的凋亡被泛半胱天冬酶抑制剂z-VAD-FMK阻断。使用抗半胱天冬酶-8和-9抗体进行免疫印迹检测到相应半胱天冬酶的激活。用3,3'-二己基氧杂羰花青碘化物染色后进行的流式细胞术分析显示线粒体膜电位丧失。我们的研究证实了BTV感染细胞中半胱天冬酶依赖性的外源性和内源性凋亡途径均参与其中。
