Liu Yi, Qiao Dai Rong, Zheng Hong Bo, Dai Xu Lan, Bai Lin Han, Zeng Jing, Cao Yi
Key Laboratory of bio-resources and Eco-environment, Ministry of Education, College of Life Sciences, Sichuan University, Sichuan, P.R. China.
Mol Biol Rep. 2008 Sep;35(3):397-403. doi: 10.1007/s11033-007-9099-x. Epub 2007 May 26.
ubiquinone oxidoreductase (complex I ) of the mitochondrial respiratory chain catalyzes the transfer of electrons from NADH to ubiquinone coupled to proton translocation across the membrane. The cDNA sequence of Dunaliella salina mitochondrial NADH: ubiquinone oxidoreductase 19-kD subunit contains a 682-bp ORF encoding a protein with an apparent molecular mass of 19 kD. The sequence has been submitted to the GenBank database under Accession No. EF566890 (cDNA sequences) and EF566891 (genomic sequence). The deduced amino-acid sequence is 74% identical to Chlamydomonas reinhardtii mitochondrial NADH:ubiquinone oxidoreductase 18-kD subunit. The 19-kD subunit mRNA expression was observed in oxygen deficiency, salt treatment, and rotenone treatment with lower levels. It demonstrate that the 19-kD subunit of Complex I from Dunaliella salina is regulated by these stresses.
线粒体呼吸链的NADH：泛醌氧化还原酶（复合体I）催化电子从NADH转移至泛醌，并偶联质子跨膜转运。盐生杜氏藻线粒体NADH：泛醌氧化还原酶19-kD亚基的cDNA序列包含一个682 bp的开放阅读框，编码一种表观分子量为19 kD的蛋白质。该序列已提交至GenBank数据库，登录号为EF566890（cDNA序列）和EF566891（基因组序列）。推导的氨基酸序列与莱茵衣藻线粒体NADH：泛醌氧化还原酶18-kD亚基的一致性为74%。在缺氧、盐处理和鱼藤酮处理中观察到19-kD亚基mRNA表达水平较低。这表明盐生杜氏藻复合体I的19-kD亚基受这些胁迫调控。
