SP1作为用于亚微米酶结构自组装纳米制造的新型支架构建块。

SP1 as a novel scaffold building block for self-assembly nanofabrication of submicron enzymatic structures.

作者信息

Heyman Arnon, Levy Ilan, Altman Arie, Shoseyov Oded

机构信息

The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel.

出版信息

Nano Lett. 2007 Jun;7(6):1575-9. doi: 10.1021/nl070450q. Epub 2007 May 26.

DOI:10.1021/nl070450q

PMID:17530810

Abstract

In this study, SP1, a ring-shaped highly stable homododecamer protein complex was utilized for the self-assembly of multiple domains in a predefined manner. Glucose oxidase (GOx) was fused in-frame to SP1 and expressed in Escherichia coli. Complexes where GOx encircled SP1 dodecamer were observed, and moreover, the enzymatic monomers self-assembled into active multienzyme nanotube particles containing hundreds of GOx molecules per tube. This work demonstrates the value of SP1 as a self-assembly scaffold.

摘要

在本研究中，利用一种环状的高度稳定的同型十二聚体蛋白复合物SP1，以预定义的方式实现多个结构域的自组装。葡萄糖氧化酶（GOx）与SP1进行读码框融合并在大肠杆菌中表达。观察到GOx环绕SP1十二聚体的复合物，此外，酶单体自组装成每管含有数百个GOx分子的活性多酶纳米管颗粒。这项工作证明了SP1作为自组装支架的价值。