Herbeck Joshua T, Lyagoba Frederick, Moore Sarah W, Shindo Nice, Biryahwaho Benon, Kaleebu Pontiano, Mullins James I
Department of Microbiology, University of Washington School of Medicine, Seattle, Washington 98195-8070, USA.
AIDS Res Hum Retroviruses. 2007 May;23(5):755-60. doi: 10.1089/aid.2006.0237.A.
To monitor the relative prevalence and evolutionary trends of HIV-1 in Uganda, we conducted a retrospective study of pregnant women over the time period 1989-2000. From a total of 300 women sampled, we defined subtypes by heteroduplex mobility assay for 230 subjects and by partial sequencing and phylogenetic analyses of the env gene for 216 subjects. Subtypes A and D were most prevalent, and there were no significant trends in relative frequencies of subtypes A (45%), D (41%), C (5%), or recombinants (9%) over the 11 years sampled. There was also no phylogenetic clustering of subtypes related to geography (clinic location) or year of collection. Mean pairwise nucleotide diversity of subtype A (pi = 0.163) and subtype D (pi =0.156) samples did not differ significantly between subtypes, nor did these levels change over the period of the study. This report suggests that among pregnant women in Uganda A and D subtypes are transmitted without geographic constraints, and are not associated with significantly different transmission rates.
为监测乌干达HIV-1的相对流行率和进化趋势，我们对1989年至2000年期间的孕妇进行了一项回顾性研究。在总共300名采样女性中，我们通过异源双链迁移分析为230名受试者确定了亚型，并通过对env基因进行部分测序和系统发育分析为216名受试者确定了亚型。A和D亚型最为普遍，在采样的11年中，A亚型（45%）、D亚型（41%）、C亚型（5%）或重组体（9%）的相对频率没有显著趋势。亚型在地理（诊所位置）或采集年份方面也没有系统发育聚类。A亚型（pi = 0.163）和D亚型（pi = 0.156）样本的平均成对核苷酸多样性在各亚型之间没有显著差异，在研究期间这些水平也没有变化。本报告表明，在乌干达孕妇中，A和D亚型的传播不受地理限制，且与显著不同的传播率无关。
