中华菝葜根茎中的3,4-二羟基苯甲酸可保护培养的大鼠皮质神经元免受β-淀粉样蛋白（25-35）诱导的神经毒性。
3,4-dihydroxybenzoic acid from Smilacis chinae rhizome protects amyloid beta protein (25-35)-induced neurotoxicity in cultured rat cortical neurons.
作者信息
Ban Ju Yeon, Cho Soon Ock, Jeon So-Young, Bae KiHwan, Song Kyung-Sik, Seong Yeon Hee
机构信息
College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk 361-763, South Korea.
出版信息
Neurosci Lett. 2007 Jun 13;420(2):184-8. doi: 10.1016/j.neulet.2007.05.009. Epub 2007 May 10.
The neuroprotective effect of 3,4-dihydroxybenzoic acid (3,4-DHBA) isolated from Smilacis chinae rhizome against Abeta (25-35)-induced neurotoxicity on cultured rat cortical neurons was found in this study. The protective effect of 3,4-DHBA against Abeta (25-35)-induced neuronal cell death was investigated by measuring cell viability via a 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) assay and Hoechst 33342 staining. 3,4-DHBA (1 and 10 microM) concentration-dependently inhibited 10 microM Abeta (25-35)-induced neuronal apoptotic death. 3,4-DHBA (1 and 10 microM) inhibited 10 microM Abeta (25-35)-induced elevation of cytosolic Ca(2+) concentration (Ca(2+)), which was measured by a fluorescent dye, Fluo-4 AM. 3,4-DHBA also inhibited glutamate release into medium, reactive oxygen species (ROS) generation, and caspase-3 activation, which were induced by 10 microM Abeta (25-35). These results suggest that 3,4-DHBA prevents Abeta (25-35)-induced neuronal cell damage by interfering with the increase of Ca(2+), and then by inhibiting glutamate release, generation of ROS and caspase-3 activity.
本研究发现，从中华菝葜根茎中分离得到的3,4-二羟基苯甲酸（3,4-DHBA）对培养的大鼠皮层神经元具有抗β淀粉样蛋白（25-35）诱导的神经毒性的神经保护作用。通过3-[4,5-二甲基噻唑-2-基]-2,5-二苯基溴化四氮唑（MTT）法和Hoechst 33342染色测量细胞活力，研究了3,4-DHBA对β淀粉样蛋白（25-35）诱导的神经元细胞死亡的保护作用。3,4-DHBA（1和10微摩尔）浓度依赖性地抑制10微摩尔β淀粉样蛋白（25-35）诱导的神经元凋亡死亡。3,4-DHBA（1和10微摩尔）抑制10微摩尔β淀粉样蛋白（25-35）诱导的胞质Ca(2+)浓度（Ca(2+)）升高，Ca(2+)通过荧光染料Fluo-4 AM测量。3,4-DHBA还抑制了由10微摩尔β淀粉样蛋白（25-35）诱导的谷氨酸释放到培养基中、活性氧（ROS）生成和半胱天冬酶-3激活。这些结果表明，3,4-DHBA通过干扰Ca(2+)的升高，进而抑制谷氨酸释放、ROS生成和半胱天冬酶-3活性，预防β淀粉样蛋白（25-35）诱导的神经元细胞损伤。
Zotero 插件