初榨橄榄油中不同的自由基清除测试及其与总酚含量的关系。

Different radical scavenging tests in virgin olive oil and their relation to the total phenol content.

作者信息

Samaniego Sánchez C, Troncoso González A M, García-Parrilla M C, Quesada Granados J J, López García de la Serrana H, López Martínez M C

机构信息

Dpto. Nutrición y Bromatología, Facultad de Farmacia, Universidad de Granada, Campus de Cartuja s/n, 18071 Granada, Spain.

出版信息

Anal Chim Acta. 2007 Jun 12;593(1):103-7. doi: 10.1016/j.aca.2007.04.037. Epub 2007 Apr 24.

DOI:10.1016/j.aca.2007.04.037

PMID:17531830

Abstract

Four different antioxidant tests (ABTS*+, DPPH, ORAC, beta-carotene-linoleate model system) were used to determine the free-radical scavenging activity of 39 extra virgin olive oils (EVOO) and compare the total phenol content by the Folin-Ciocalteu method. The correlation between the total phenols and antioxidant capacities measured by the four methods was very high, and highest with ABTS*+ (R2 = 0.9905). Some of these methods of measurement were applied to olive-oil samples (OO), with approximately a 50% decrease in the value of the antioxidant capacity in comparison with values found for EVOO. In conclusion, the results show that all the methods tested were suitable for determining the antioxidant capacity of olive oil. The Picual variety of extra-virgin olive oil showed high antioxidant activity.

摘要

采用四种不同的抗氧化剂测试方法（ABTS*+、DPPH、ORAC、β-胡萝卜素-亚油酸酯模型体系）来测定39种特级初榨橄榄油（EVOO）的自由基清除活性，并通过福林-西奥尔特法比较其总酚含量。四种方法测得的总酚与抗氧化能力之间的相关性非常高，其中与ABTS*+的相关性最高（R2 = 0.9905）。将其中一些测量方法应用于橄榄油样品（OO）时，其抗氧化能力值与特级初榨橄榄油相比下降了约50%。总之，结果表明所有测试方法都适用于测定橄榄油的抗氧化能力。皮夸尔品种的特级初榨橄榄油表现出较高的抗氧化活性。