一种用于鉴别幼年斑胸草雀性别的微创手术。
A minimally invasive procedure for sexing young zebra finches.
作者信息
Soderstrom Ken, Qin Weixi, Leggett Matthew H
机构信息
Department of Pharmacology and Toxicology, Brody School of Medicine, East Carolina University, Greenville, NC 27834, USA.
出版信息
J Neurosci Methods. 2007 Aug 15;164(1):116-9. doi: 10.1016/j.jneumeth.2007.04.007. Epub 2007 Apr 18.
Abstract
Zebra finches have been widely used to study neurobiology underlying vocal development. Because only male zebra finches learn song, efficient developmental use of these animals requires early determination of sex at ages that precede maturation of secondary sex characteristics. We have developed a sex determination method that combines a forensics method of genomic DNA isolation (from very small blood samples) with PCR amplification from Z and W sex chromosomes (males are ZZ, females ZW). This combination results in a minimally invasive yet highly reliable and convenient genotyping method.
摘要
斑胸草雀已被广泛用于研究发声发育背后的神经生物学。由于只有雄性斑胸草雀学习鸣叫，要有效利用这些动物进行发育研究，需要在第二性征成熟之前的年龄尽早确定性别。我们开发了一种性别鉴定方法，该方法将基因组DNA分离的法医方法（从非常少量的血液样本中）与Z和W性染色体的PCR扩增相结合（雄性为ZZ，雌性为ZW）。这种结合产生了一种微创但高度可靠且方便的基因分型方法。
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