萝卜花叶病毒日本分离株的基因组序列：首个十字花科感染豇豆花叶病毒属病毒的完整核苷酸序列
Genome sequence of a Japanese isolate of Radish mosaic virus: the first complete nucleotide sequence of a crucifer-infecting comovirus.
作者信息
Komatsu K, Hashimoto M, Maejima K, Ozeki J, Kagiwada S, Takahashi S, Yamaji Y, Namba S
机构信息
Laboratory of Plant Pathology, Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
出版信息
Arch Virol. 2007;152(8):1501-6. doi: 10.1007/s00705-007-0993-2. Epub 2007 May 29.
The complete nucleotide sequences of RNA1 and RNA2 of a Japanese isolate of Radish mosaic virus (RaMV-J), a crucifer-infecting comovirus, were determined. RNA1 is 6064 nucleotides long and encodes a 210-kDa polyprotein containing conserved motifs that are required for replication. RNA2 is 4020 nucleotides long and encodes a 123-kDa polyprotein containing the putative movement protein and two coat proteins. Comparisons of the encoded proteins confirmed that RaMV-J and a Czech RaMV isolate are isolates of the same species in the genus Comovirus. A phylogenetic analysis of RaMV-J and other comoviruses revealed that legume-infecting comoviruses constitute a single branch to which RaMV is distantly related.
测定了萝卜花叶病毒日本分离株（RaMV-J）的RNA1和RNA2的完整核苷酸序列，该病毒是一种感染十字花科植物的豇豆花叶病毒属病毒。RNA1长6064个核苷酸，编码一个210 kDa的多蛋白，该多蛋白含有复制所需的保守基序。RNA2长4020个核苷酸，编码一个123 kDa的多蛋白，该多蛋白含有假定的运动蛋白和两个外壳蛋白。对编码蛋白的比较证实，RaMV-J和捷克的RaMV分离株是豇豆花叶病毒属同一物种的分离株。对RaMV-J和其他豇豆花叶病毒的系统发育分析表明，感染豆科植物的豇豆花叶病毒构成一个单一支系，RaMV与之亲缘关系较远。
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