Barcew K, Paczkowska E, Dabkowska E, Baskiewicz-Masiuk M, Marchlewicz M, Domański L, Sagan L, Wiszniewska B, Machaliński B
Department of General Pathology, Pomeranian Medical University, Szczecin, Poland.
Folia Morphol (Warsz). 2007 Feb;66(1):1-9.
The cellular mobilisation of mice with granulocyte colony-stimulating factor (G-CSF) results in an egress of haematopoietic stem/progenitor cells from the bone marrow and an increase in their level in the peripheral blood. While the mobilisation process with different agents is widely studied, little is known about the morphology of the murine haematopoietic organs during the mobilisation. The purpose of this study was to examine the morphology of the bone marrow, spleen and liver in mice mobilised with G-CSF. To address this issue mice were injected subcutaneously with G-CSF for 6 consecutive days. Morphological analysis revealed an increase in the number of mature neutrophils close to the wall of sinusoids in the bone marrow as well as hypertrophy of the red pulp in the spleen. At the same time no morphological changes were noticed in the livers of G-CSF-mobilised mice. In conclusion, G-CSF induces discrete ultrastructural changes in the bone marrow, which intensify the transendothelial traverse of haematopoietic stem and progenitor cells from it. The changes in the spleen are related to repopulation of this organ by mobilised early haematopoietic cells circulating in the peripheral blood. We also noticed that the process of migration of haematopoietic cells from the bone marrow into the peripheral blood began on day 2 and was most pronounced on day 4 after stimulation with G-CSF.
用粒细胞集落刺激因子（G-CSF）动员小鼠会导致造血干/祖细胞从骨髓中流出，并使其在外周血中的水平升高。虽然对不同药物的动员过程进行了广泛研究，但对于动员过程中小鼠造血器官的形态了解甚少。本研究的目的是检查用G-CSF动员的小鼠的骨髓、脾脏和肝脏的形态。为解决这个问题，给小鼠连续6天皮下注射G-CSF。形态学分析显示，骨髓中靠近血窦壁的成熟中性粒细胞数量增加，脾脏红髓肥大。同时，在G-CSF动员的小鼠肝脏中未观察到形态学变化。总之，G-CSF诱导骨髓中离散的超微结构变化，这增强了造血干细胞和祖细胞从骨髓中的跨内皮迁移。脾脏的变化与外周血中循环的早期造血细胞动员后该器官的重新填充有关。我们还注意到，造血细胞从骨髓迁移到外周血的过程在第2天开始，在用G-CSF刺激后第4天最为明显。
