Fiorani Celedon Paola Alejandra, de Andrade Alexander, Meireles Karem Guimarães Xavier, Gallo de Carvalho Mayra Costa da Cruz, Caldas Danielle Gregorio Gomes, Moon David Henry, Carneiro Raphael Tozelli, Franceschini Lívia Maria, Oda Shinitiro, Labate Carlos Alberto
Laboratório Max Feffer de Genética de Plantas, Departamento de Genética, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, Piracicaba-SP, Brazil.
Proteomics. 2007 Jun;7(13):2258-74. doi: 10.1002/pmic.200600989.
Recent advances in genomics and proteomics have provided an excellent opportunity to understand complex biological processes such as wood formation at the gene and protein levels. The aim of this work was to describe the proteins participating in the processes involved in juvenile wood formation by isolating proteins from the cambial region of Eucalyptus grandis, at three ages of growth (6-month-old seedlings, 3- and 6-year-old trees), and also to identify proteins differentially expressed. Using a 2-D-LC-MS/MS strategy we identified a total of 240 proteins, with 54 corresponding spots being present in at least two ages. Overall, nine proteins classified into the functional categories of metabolism, cellular processes, and macromolecular metabolism showed significant changes in expression. Proteins were classified into seven main functional categories, with metabolism representing 35.2% of the total proteins identified. The comparison of the reference maps showed not only differences in the expression pattern of individual proteins at each age, but also among isoforms. The results described in this paper provide a dynamic view of the proteins involved in the formation of juvenile wood in E. grandis.
基因组学和蛋白质组学的最新进展为在基因和蛋白质水平上理解复杂的生物学过程（如木材形成）提供了绝佳机会。这项工作的目的是通过从大桉木形成层区域分离蛋白质，描述参与幼龄木材形成过程的蛋白质，分离对象为三个生长年龄（6个月大的幼苗、3年和6年树龄的树木），同时鉴定差异表达的蛋白质。使用二维液相色谱-串联质谱策略，我们总共鉴定出240种蛋白质，其中54个相应斑点至少在两个年龄中出现。总体而言，九种归类于代谢、细胞过程和大分子代谢功能类别的蛋白质表达出现显著变化。蛋白质被分为七个主要功能类别，代谢类占鉴定出的总蛋白质的35.2%。参考图谱的比较不仅显示了每个年龄个体蛋白质表达模式的差异，也显示了同工型之间的差异。本文所述结果提供了大桉木幼龄木材形成过程中涉及蛋白质的动态视图。
