Wang Yan-hua, Wang Yi, Wang Dong-wu, Wu Nan, Liu Dong-ning, Yin Zheng-qin
Department of Ophthalmology, Southwest Hospital, Third Military Medical University, Chongqing, China.
Ophthalmic Res. 2007;39(3):174-8. doi: 10.1159/000103237. Epub 2007 May 25.
RhoA is a small guanosine triphosphatase which participates in signaling pathways of axonal repellents or inhibitors. However, the distribution and expression of RhoA in the rat retina after optic nerve injury has not been elucidated yet.
To study the distribution and expression of RhoA in the rat retina after optic nerve injury.
Immunohistochemistry was used to determine the distribution of RhoA in rat retina after optic nerve injury. The expression of RhoA was analyzed by Western blot.
In normal retina and the retina 1 day after optic nerve injury, RhoA was distributed in the retinal ganglion cell (RGC) layer. Three days after optic nerve injury, it existed in RGCs and the inner plexiform layer. However, 7 days after surgery its immunoreactivity was abundant not only in the RGC and inner plexiform layers but also in the inner nuclear and outer plexiform layers. Western blot analysis showed that the expression of RhoA increased significantly in the retina after optic nerve injury in comparison with normal retina.
These results indicate that the distribution and expression of RhoA were extended and enhanced after optic nerve injury, and that RhoA plays an important role in optic nerve regeneration.
RhoA是一种小GTP酶，参与轴突排斥因子或抑制剂的信号通路。然而，视神经损伤后RhoA在大鼠视网膜中的分布和表达尚未阐明。
研究视神经损伤后RhoA在大鼠视网膜中的分布和表达。
采用免疫组织化学法确定视神经损伤后RhoA在大鼠视网膜中的分布。通过蛋白质免疫印迹法分析RhoA的表达。
在正常视网膜和视神经损伤后1天的视网膜中，RhoA分布于视网膜神经节细胞（RGC）层。视神经损伤后3天，它存在于RGC和内网状层。然而，术后7天，其免疫反应性不仅在RGC和内网状层丰富，而且在内核层和外网状层也丰富。蛋白质免疫印迹分析表明，与正常视网膜相比，视神经损伤后视网膜中RhoA的表达显著增加。
这些结果表明，视神经损伤后RhoA的分布和表达扩展并增强，且RhoA在视神经再生中起重要作用。
