Grueterich Martin, Kenyon Kenneth R, Priglinger Siegfried, Welge-Luessen Ulrich, Lackerbauer Carlo, Kampik A
Department of Ophthalmology, Ludwig Maximilians University, Munich, Germany.
Ophthalmic Res. 2007;39(3):179-83. doi: 10.1159/000103238. Epub 2007 May 25.
To perform lamellar keratolimbal allograft transplantation in a one-step procedure with a single graft, we investigated the feasibility of harvesting eccentric lamellar keratolimbal grafts from conventionally processed corneoscleral buttons using a manually guided microkeratome in conjunction with an artificial anterior chamber system.
We used the Moria LSK-One microkeratome and the automated lamellar therapeutic keratoplasty (ALTK) system (Antony, France). Ten human donor eyes were used to obtain single-piece lamellar keratolimbal grafts. Specimens were processed for light and electron microscopy.
Eccentric keratolimbal grafts could be obtained from all human donor buttons. Grafts include a crescent-shaped limbal and a large corneal portion. No visible damage to the limbal region was discernible.
Our data show that the LSK-One microkeratome in conjunction with the ALTK system allows harvesting eccentric keratolimbal grafts from donor corneoscleral buttons.
为了采用单块移植片一步法进行板层角膜缘移植，我们研究了使用手动引导微型角膜刀结合人工前房系统，从常规处理的角膜巩膜纽扣中获取偏心板层角膜缘移植片的可行性。
我们使用了Moria LSK-One微型角膜刀和自动板层治疗性角膜移植术（ALTK）系统（法国安东尼）。使用10只人类供体眼获取单片板层角膜缘移植片。对标本进行光镜和电镜处理。
可以从所有人类供体纽扣中获得偏心角膜缘移植片。移植片包括新月形角膜缘和大片角膜部分。角膜缘区域未见明显损伤。
我们的数据表明，LSK-One微型角膜刀结合ALTK系统能够从供体角膜巩膜纽扣中获取偏心角膜缘移植片。
