Kim Mi-Sun, Kim Hyun-Soon, Kim Yoon-Shik, Baek Kwang-Hyun, Oh Hyun-Woo, Hahn Kyu-Woong, Bae Ro-Na, Lee In-Jung, Joung Hyouk, Jeon Jae-Heung
Plant Genome Research Center, KRIBB, Oun 52, Yuseong, Daejeon, 305-806, South Korea.
Plant Cell Rep. 2007 Oct;26(10):1717-25. doi: 10.1007/s00299-007-0380-1. Epub 2007 May 30.
A higher concentration of H2O2 was detected in the sense transgenic potato plant (SS4) with the lily chCu,ZnSOD sequence, whereas higher levels of O2(-) was detected in the antisense transgenic plant (SA1) than the WT plant. The elongation growth in SA1 was significantly inhibited by treatment with diphenyleneiodonium, an inhibitor of O2(-) generation, and promoted in the SS4 on treatment with herbicide methyl viologen, a generator of apoplastic O2(-) . Higher concentrations of GAs were detected during plant growth and the early stage of tuberization in SA1. Complete recovery of the above elongation growth and microtuberization pattern in transgenic plants following treatment of GA(3) or an inhibitor of gibberellin synthesis, paclobutrazol, indicate that these changes were mainly caused by active GA levels. In conclusion, a specific ROS (O2(-) ) acts as a signal transducer via GA biosynthetic pathways for the regulation of plant growth and tuber development of potato.
在含有百合chCu,ZnSOD序列的有义转基因马铃薯植株（SS4）中检测到较高浓度的H2O2，而在反义转基因植株（SA1）中检测到的O2(-)水平高于野生型植株。用O2(-)生成抑制剂二苯基碘鎓处理后，SA1的伸长生长受到显著抑制，而用质外体O2(-)生成剂除草剂甲基紫精处理后，SS4的伸长生长得到促进。在SA1的植株生长和块茎形成早期检测到较高浓度的赤霉素。用GA(3)或赤霉素合成抑制剂多效唑处理后，转基因植株上述伸长生长和微型块茎形成模式完全恢复，表明这些变化主要是由活性赤霉素水平引起的。总之，一种特定的活性氧（O2(-)）通过赤霉素生物合成途径作为信号转导分子，调控马铃薯的植株生长和块茎发育。
