Tao J, Zhang X-P, Chen X P, Li Y, Liu Y Q, Tian J, Huang C-Z, Shen G-X, Tu Y-T
Department of Dermatology, Affiliated Union Hospital, Huazhong University of Science and Technology, Wuhan, China.
Clin Exp Dermatol. 2007 Sep;32(5):550-5. doi: 10.1111/j.1365-2230.2007.02455.x. Epub 2007 May 29.
Condyloma acuminatum (CA), caused by human papillomavirus (HPV), is characterized by a variable clinical course that can include significant morbidity, frequent disease recurrence and occasional oncogenicity. Effective CD8+ T-cell-mediated clearance of HPV-infected cells may be defective in patients with CA, leading to recurrent disease and failure to suppress latent HPV reactivation. The pathogenesis responsible for CA and the persistence of latent HPV infection remain unknown.
To determine whether expression of transporters associated with antigen processing 1 (TAP-1) and the major histocompatibility complex class I (MHC-I) is involved in HPV immune escape.
In this present study, we compared 31 CA lesions with 30 normal prepuces by immunohistochemistry and reverse transcription PCR for their expressions of TAP-1 and MHC-I.
Expressions of TAP-1 and MHC-I were significantly reduced in CA tissue biopsies compared with normal prepuces. There was a statistically significant positive correlation between expressions of TAP-1 and MHC-I in CA lesions. Furthermore, we found that TAP-1 mRNA was significantly reduced in CA lesions compared with those in normal prepuces.
These results suggest that HPV may evade immune recognition by downregulating MHC-I cell surface expression via decreased TAP-1 levels.
尖锐湿疣（CA）由人乳头瘤病毒（HPV）引起，其临床病程多变，可包括严重的发病率、疾病频繁复发以及偶尔的致癌性。在尖锐湿疣患者中，有效的CD8 + T细胞介导的HPV感染细胞清除功能可能存在缺陷，导致疾病复发以及无法抑制潜伏HPV的重新激活。导致尖锐湿疣和潜伏HPV感染持续存在的发病机制仍不清楚。
确定与抗原加工相关的转运体1（TAP-1）和主要组织相容性复合体I类（MHC-I）的表达是否参与HPV免疫逃逸。
在本研究中，我们通过免疫组织化学和逆转录PCR比较了31个尖锐湿疣病变组织和30个正常包皮组织中TAP-1和MHC-I的表达。
与正常包皮相比，尖锐湿疣组织活检中TAP-1和MHC-I的表达显著降低。尖锐湿疣病变中TAP-1和MHC-I的表达之间存在统计学上显著的正相关。此外，我们发现与正常包皮相比，尖锐湿疣病变中TAP-1 mRNA显著降低。
这些结果表明，HPV可能通过降低TAP-1水平下调MHC-I细胞表面表达来逃避免疫识别。
