[热缺血再灌注损伤对肝脏细胞周期调控的影响]
[The influence of warm ischemia-reperfusion injury on cell cycle control in liver].
作者信息
Zhang Shao, He Xiao-shun, Ma Yi, Wang Dong-ping, Ju Wei-qiang, Wu Lin-wei, Zhu Xiao-feng, Huang Jie-fu
机构信息
Organ Transplantation Center, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China.
出版信息
Zhonghua Wai Ke Za Zhi. 2007 Mar 1;45(5):335-8.
OBJECTIVE
To observe the effect of warm ischemia-reperfusion injury (WIRI) on the genes involved in the cell cycle in liver and investigate the molecular mechanism of WIRI.
METHODS
Twenty-four Sprague-Dawley male rats were divided into experiment groups and sham operation group respectively. According to the dynamic characteristics, the genes involved in the cell cycle were submitted for multivariate cluster and self-organized map analysis. Total RNA was extracted for the microarray analysis and subsequent real-time quantitative PCR.
RESULTS
There were 86, 410, 234 up-regulated genes and 136, 312, 653 down-regulated genes over 2 - 4 times in the 0 h, 1 h and 24 h group respectively, most of which such as Gadd45a, Egr1, Hsp70 were involved in the cell cycle checkpoint, energy metabolism, signal transduction, immune-associated procedure, and so on. In addition, the results of microarray analysis were confirmed by real-time quantitative PCR.
CONCLUSIONS
Some genes related with cell cycle and regeneration were activated in the early stage of WIRI. Gadd45a, Egr1 and Hsp70 may be new targets to cure WIRI.
目的
观察肝脏热缺血再灌注损伤（WIRI）对细胞周期相关基因的影响，探讨WIRI的分子机制。
方法
将24只雄性Sprague-Dawley大鼠分为实验组和假手术组。根据动态特征，对细胞周期相关基因进行多变量聚类和自组织映射分析。提取总RNA进行微阵列分析及后续实时定量PCR。
结果
0 h、1 h和24 h组分别有86、410、234个基因上调2 - 4倍以上，136、312、653个基因下调2 - 4倍以上，其中Gadd45a、Egr1、Hsp70等多数基因参与细胞周期检查点、能量代谢、信号转导、免疫相关过程等。此外，微阵列分析结果经实时定量PCR验证。
结论
WIRI早期一些与细胞周期和再生相关的基因被激活。Gadd45a、Egr1和Hsp70可能是治疗WIRI的新靶点。
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