Kent Rebekah J, Mharakurwa Sungano, Hamapumbu Harry, Norris Douglas E
The W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Malaria Research Institute, Johns Hopkins Bloomberg School of Public Health, 615 N Wolfe Street, Baltimore, MD 21205, USA.
J Am Mosq Control Assoc. 2007 Mar;23(1):71-5. doi: 10.2987/8756-971X(2007)23[71:ROANMM]2.0.CO;2.
Genetic mutations controlling eye color, fat body color, structural abnormalities, and insecticide resistance are common in mosquitoes. We have identified a novel color variant of Culex pipiens quinquefasciatus characterized by a heavily pigmented integument in adult specimens circulating in field populations of this species in southern Zambia. Mosquitoes were collected monthly by pyrethrum spray catch between November and May 2004-05 and 2005-06, with between 25% and 80% of the total Cx. p. quinquefasciatus collections comprising this pigmented variety. The identity of pigmented specimens was morphologically confirmed as Cx. p. quinquefasciatus by examination of orientation of the dorsal and ventral arms of the male genitalia of F1 male progeny reared in the laboratory, and molecularly verified by diagnostic polymerase chain reaction and nicotinamide adenine dinucleotide phosphate dehydrogenase subunit 4 nucleotide sequence homology. Preliminary laboratory rearings indicated that the pigmentation was heritable and not influenced by larval habitat and environmental conditions. Further investigation into the mechanism of the mutation, inheritance patterns, and potential linkage to additional markers is pending.
控制眼睛颜色、脂肪体色、结构异常和抗杀虫剂的基因突变在蚊子中很常见。我们已经鉴定出一种新型的致倦库蚊颜色变体，其特征是在赞比亚南部该物种野外种群中循环的成年标本中体表色素沉着严重。在2004 - 05年和2005 - 06年的11月至5月期间，每月通过除虫菊酯喷雾捕获法收集蚊子，所收集的致倦库蚊总数中有25%至80%为这种有色素的变种。通过检查在实验室饲养的F1雄性后代雄性生殖器背臂和腹臂的方向，从形态学上确认有色素标本为致倦库蚊，并通过诊断性聚合酶链反应和烟酰胺腺嘌呤二核苷酸磷酸脱氢酶亚基4核苷酸序列同源性进行分子验证。初步的实验室饲养表明，色素沉着是可遗传的，不受幼虫栖息地和环境条件的影响。关于该突变机制、遗传模式以及与其他标记潜在联系的进一步研究正在进行中。
