Skoneczna Adrianna
Pracownia Mutagenezy i Reperacji DNA, Instytut Biochemii i Biofizyki PAN, Warszawa.
Postepy Biochem. 2006;52(4):435-47.
The last ten years, since yeast Saccharomyces cerevisiae genome was sequenced, brought a big impact in genome-wide techniques. The tenth anniversary of genomic era provokes the following resume: a lot of new methods were invented. The yeast strains libraries carrying transposon insertions, gene deletions or tagged proteins have been created. Using them, the phenotypes of gene deletions, as well as the biological activity, cellular localization and possible modifications of their protein products were elucidated. SAGE analysis and DNA microarray experiments showed gene expression profiles and allowed to build interaction networks of gene regulation. The two dimensional gels, mass spectrometry, protein arrays and two-hybrid system carry information about protein interactions, modifications, and biochemical activities. All these methods permit to increase the number of genes with known cellular functions. Moreover, testing these techniques on yeast S. cerevisiae--a model eukaryotic organism--opened the door for their usage in all other species.
自酿酒酵母基因组测序以来的过去十年，给全基因组技术带来了巨大影响。基因组时代十周年引发了如下回顾：发明了许多新方法。构建了携带转座子插入、基因缺失或标记蛋白的酵母菌株文库。利用这些文库，阐明了基因缺失的表型及其蛋白产物的生物学活性、细胞定位和可能的修饰。SAGE分析和DNA微阵列实验展示了基因表达谱，并得以构建基因调控的相互作用网络。二维凝胶电泳、质谱分析、蛋白质阵列和双杂交系统携带了有关蛋白质相互作用、修饰和生化活性的信息。所有这些方法都有助于增加具有已知细胞功能的基因数量。此外，在模式真核生物酿酒酵母上测试这些技术，为它们在所有其他物种中的应用打开了大门。
