Li Peng, Li Shao-Ping, Yang Feng-Qing, Wang Yi-Tao
Institute of Chinese Medical Sciences, University of Macau, Taipa, Macau, China.
J Sep Sci. 2007 Apr;30(6):900-5. doi: 10.1002/jssc.200600368.
A pressurized liquid extraction (PLE) and CEC were developed for the simultaneous determination of four tanshinones (dihydrotanshinone I, cryptotanshinone, tanshinone I, and tanshinone IIA) in Salvia miltiorrhiza. High extraction efficiency (>98.5%) was achieved under the optimum PLE conditions. A good separation was obtained by using a Hypersil C18 capillary (3 microm, 100 microm/25 cm) with a mixture of 30 mM Tris-HCl (pH 8.5)-ACN (1:3, v/v) as BGE solution running at 20 kV and 20 degrees C within 12 min. All the calibration curves showed good linearity (r2 >0.9958) within test ranges. The developed method showed good repeatability for the quantification of four investigated components in S. miltiorrhiza with intra- and interday variations of less than 4.4 and 6.8%, respectively. The validated method was successfully applied to quantify four tanshinones in S. miltiorrhiza, which is helpful to control the quality of S. miltiorrhiza.
建立了一种加压液体萃取（PLE）和毛细管电色谱（CEC）方法，用于同时测定丹参中四种丹参酮（二氢丹参酮I、隐丹参酮、丹参酮I和丹参酮IIA）。在最佳PLE条件下实现了高萃取效率（>98.5%）。使用Hypersil C18毛细管（3μm，100μm/25 cm），以30 mM Tris-HCl（pH 8.5）-乙腈（1:3，v/v）的混合物作为背景电解质溶液，在20 kV和20℃下运行12分钟，获得了良好的分离效果。所有校准曲线在测试范围内均显示出良好的线性（r2>0.9958）。所建立的方法对丹参中四种被研究成分的定量具有良好的重复性，日内和日间变化分别小于4.4%和6.8%。该验证方法成功应用于丹参中四种丹参酮的定量分析，有助于控制丹参的质量。
