Shi Zhihong, He Jiantao, Yao Tingting, Chang Wenbao, Zhao Meiping
Institute of Analytical Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, PR China.
J Pharm Biomed Anal. 2005 Mar 9;37(3):481-6. doi: 10.1016/j.jpba.2004.11.015. Epub 2004 Dec 23.
A reversed-phase high performance liquid chromatographic method was established for the simultaneous determination of tanshinones in five kinds of traditional Chinese medicinal preparations (TCMPs) containing Radix salvia miltiorrhiza (Chinese herbal name: Danshen). Tanshinones including cryptotanshinone, tanshinone I and tanshinone IIA were successfully separated on a Diamonsil C18 column (150 mm x 4.6 mm i.d., 5 microm). The mobile phase was a mixture of methanol, tetrahydrofuran, water and glacial acetic acid (20:35:44:1, v/v/v/v), employing isocratic elution at a flow rate of 1.0 mL/min. Detection was accomplished at 254 nm. The compounds were identified by comparing their retention times and UV spectra in the 200-400 nm range with authentic standards. Regression equations revealed good linear relationship between the peak areas of the constituents and their concentrations (correlation coefficients: 0.9998 for cryptotanshinone, 0.9999 for tanshinone I and 1.0000 for tanshinone IIA). The relative standard deviations (n=6) of retention time and peak area were less than 0.25% and 1.00%, respectively. The recoveries were between 96.2% and 102.5%. The proposed method has been successfully applied to the simultaneous determination of the tanshinones in five kinds of Chinese herbal preparations containing Danshen within 20 min.
建立了一种反相高效液相色谱法，用于同时测定五种含丹参的传统中药制剂中的丹参酮。隐丹参酮、丹参酮Ⅰ和丹参酮ⅡA等丹参酮在Diamonsil C18柱（150 mm×4.6 mm内径，5μm）上成功分离。流动相为甲醇、四氢呋喃、水和冰醋酸的混合物（20:35:44:1，v/v/v/v），采用等度洗脱，流速为1.0 mL/min。检测波长为254 nm。通过将其保留时间和200 - 400 nm范围内的紫外光谱与标准品进行比较来鉴定化合物。回归方程显示各成分的峰面积与其浓度之间具有良好的线性关系（相关系数：隐丹参酮为0.9998，丹参酮Ⅰ为0.9999，丹参酮ⅡA为1.0000）。保留时间和峰面积的相对标准偏差（n = 6）分别小于0.25%和1.00%。回收率在96.2%至102.5%之间。所建立的方法已成功应用于在20分钟内同时测定五种含丹参的中药制剂中的丹参酮。
