Stagos Dimitrios, Spanou Chrysa, Margariti Maria, Stathopoulos Constantinos, Mamuris Zissis, Kazantzoglou Georgios, Magiatis Prokopios, Kouretas Demetrios
Department of Biochemistry and Biotechnology, University of Thessaly, Ploutonos 26 & Aiolou, GR-41221 Larissa, Greece.
J Agric Food Chem. 2007 Jun 27;55(13):5246-52. doi: 10.1021/jf0635255. Epub 2007 May 31.
In the present study, the effects of extracts and polyphenol-rich fractions as well as monomer polyphenols identified in them, from both red and white grapes, on mitomycin C (MMC) induced sister chromatid exchanges (SCEs) in human peripheral blood lymphocytes were investigated. The grape extracts and two of the three polyphenol-rich fractions promoted MMC-induced SCEs at concentrations from 75 to 300 microg/mL. However, none of the extracts or fractions alone induced SCEs. Thus, these results suggest caution especially with regard to the use of grape extracts as dietary supplements. On the other hand, the fact that these extracts were not genotoxic alone may indicate a selective activity against genetically damaged cells. This is the first study regarding the clastogenic effects of grape extracts in human cells. Moreover, from the tested polyphenols, caffeic acid, gallic acid, and rutin hydrate enhanced MMC-induced clastogenicity, whereas ferulic acid, protocatechuic acid, (+)-catechin, (-)-epicatechin, and trans-resveratrol had no effect at concentrations between 5 and 100 microM. The differences in the chemical structures of the tested polyphenols may account for their differential effects on MMC clastogenicity.
在本研究中，研究了红葡萄和白葡萄中的提取物、富含多酚的组分以及其中鉴定出的单体多酚对丝裂霉素C（MMC）诱导的人外周血淋巴细胞姐妹染色单体交换（SCE）的影响。葡萄提取物和三个富含多酚的组分中的两个在浓度为75至300微克/毫升时促进了MMC诱导的SCE。然而，单独的提取物或组分均未诱导SCE。因此，这些结果表明尤其在将葡萄提取物用作膳食补充剂时需谨慎。另一方面，这些提取物单独无遗传毒性这一事实可能表明其对基因受损细胞具有选择性活性。这是关于葡萄提取物对人细胞致断裂作用的首次研究。此外，在所测试的多酚中，咖啡酸、没食子酸和芦丁水合物增强了MMC诱导的断裂作用，而阿魏酸、原儿茶酸、（+）-儿茶素、（-）-表儿茶素和反式白藜芦醇在5至100微摩尔浓度下无作用。所测试多酚化学结构的差异可能解释了它们对MMC断裂作用的不同影响。
