Glaeser Jens, Zobawa Monica, Lottspeich Friedrich, Klug Gabriele
Institut für Mikrobiologie und Molekularbiologie, Justus-Liebig-Universität Giessen, Heinrich-Buff-Ring 26-32, D-35392 Giessen, Germany.
J Proteome Res. 2007 Jul;6(7):2460-71. doi: 10.1021/pr060624p. Epub 2007 May 31.
Singlet oxygen (1O2) is a stress factor and signal in the facultative phototrophic bacterium Rhodobacter sphaeroides. In vivo protein labeling with L-[35S]-methionine and analysis by two-dimensional gel electrophoresis revealed that the synthesis of 61 proteins was changed in response to 1O2. After 1O2 treatment, protein synthesis patterns were distinct from those after H2O2 treatment but similar to those after high light exposure. This indicates regulatory mechanisms selective for different reactive oxygen species (ROS) and a response to light partly mediated by 1O2. Analysis of mutant strains support that the response to 1O2 is regulated mainly by rpoE (sigma E), but also a modulation of the sigma E dependent response by other factors and the existence of sigma E independent responses. The involvement of the RNA chaperon Hfq in the 1O2 response implies a role of small regulatory RNAs.
单线态氧（1O2）是兼性光合细菌球形红杆菌中的一种应激因子和信号。用L-[35S]-甲硫氨酸进行体内蛋白质标记并通过二维凝胶电泳分析表明，61种蛋白质的合成因1O2而发生变化。1O2处理后，蛋白质合成模式与H2O2处理后的不同，但与高光照射后的相似。这表明对不同活性氧（ROS）具有选择性的调控机制以及部分由1O2介导的对光的响应。对突变菌株的分析支持，对1O2的响应主要由rpoE（σE）调控，但其他因素也对σE依赖性响应有调节作用，并且存在不依赖σE的响应。RNA伴侣蛋白Hfq参与1O2响应意味着小调节RNA发挥了作用。
