Zappala Christopher, Chandan Snehal, George Narelle, Faoagali Joan, Boots Robert J
Department of Intensive Care Medicine, Royal Brisbane and Women's Hospital, Brisbane, QLD, Australia.
Crit Care Resusc. 2007 Jun;9(2):157-60.
The mucolytic, anticoagulative, anti-inflammatory and neo-angiogenic properties of inhaled heparin may benefit patients with burns and cystic fibrosis. We assessed the antibacterial effects of unfractionated heparin.
Stored clinical isolates of Acinetobacter baumannii (n =4), Candida albicans (n = 5), Haemophilus influenzae (n =5), Klebsiella pneumoniae (n =4), methicillin-resistant Staphylococcus aureus (n=3), Pseudomonas aeruginosa (n = 2), and Streptococcus pneumoniae (n = 7) were subcultured on horse blood agar, incubated at 35 degrees C overnight, then inoculated into trypticase soy broth to a density of 1 McFarland standard. Dilutions of unfractionated heparin (containing 250- 7500 U) and 100 microL of the 1.0 McFarland standard broth were incubated at 35 degrees C overnight in microtitre plates and then subcultured on horse blood agar using 1 microL standard loops. Colonies (representing viable organisms) were counted.
Heparin produced dose-dependent growth inhibition of three of seven S. pneumoniae isolates (complete inhibition at 2500U dose per 200 microL) and one of five H. influenzae isolates (complete inhibition at 7500 U dose per 200 microL), but no inhibition of other isolates.
Unfractionated heparin is unlikely to have antibacterial effects because of its unpredictable inhibition of growth of common respiratory pathogens.
吸入性肝素的黏液溶解、抗凝、抗炎和促血管生成特性可能对烧伤和囊性纤维化患者有益。我们评估了普通肝素的抗菌作用。
将储存的鲍曼不动杆菌（n = 4）、白色念珠菌（n = 5）、流感嗜血杆菌（n = 5）、肺炎克雷伯菌（n = 4）、耐甲氧西林金黄色葡萄球菌（n = 3）、铜绿假单胞菌（n = 2）和肺炎链球菌（n = 7）的临床分离株在马血琼脂上进行传代培养，于35℃孵育过夜，然后接种到胰蛋白酶大豆肉汤中，使其密度达到1麦氏标准。将普通肝素（含250 - 7500单位）的稀释液和100微升1.0麦氏标准肉汤在微量滴定板中于35℃孵育过夜，然后使用1微升标准接种环接种到马血琼脂上。对菌落（代表活菌）进行计数。
肝素对7株肺炎链球菌分离株中的3株产生了剂量依赖性生长抑制（每200微升2500单位剂量时完全抑制），对5株流感嗜血杆菌分离株中的1株产生了剂量依赖性生长抑制（每200微升7500单位剂量时完全抑制），但对其他分离株无抑制作用。
普通肝素不太可能具有抗菌作用，因为其对常见呼吸道病原体生长的抑制作用不可预测。
