Janos Pavel, Zatrepálková Ivana
University of Jan Evangelista Purkyne, Faculty of the Environment, Králova Výsina 7, 400 96 Ustí nad Labem, Czech Republic.
J Chromatogr A. 2007 Aug 10;1160(1-2):160-5. doi: 10.1016/j.chroma.2007.05.027. Epub 2007 May 16.
HEMA, the copolymer of 2-hydroxyethyl methacrylate and ethylene dimethacrylate, has proven to be a suitable stationary phase for the size-exclusion chromatographic (SEC) characterization of humic substances in a wide pH range. The SEC measurements were performed typically with alkaline borate-based mobile phase: 0.025 mol/l Na2B4O7, 0.025 mol/l NaCl, 0.001 mol/l Na4P2O7, pH 9.1. After calibrating with globular protein standards, apparent molecular weights of typical peat- and coal-derived humic acids were estimated. The average molecular weights determined with the aid of the HEMA column were quite comparable with the values obtained previously by the method of low-pressure gel-permeation chromatography on the Sephadex column.
甲基丙烯酸2-羟乙酯（HEMA），即甲基丙烯酸2-羟乙酯与二甲基丙烯酸乙二酯的共聚物，已被证明是一种适用于在较宽pH范围内对腐殖质进行尺寸排阻色谱（SEC）表征的固定相。SEC测量通常使用碱性硼酸盐基流动相进行：0.025 mol/l Na2B4O7、0.025 mol/l NaCl、0.001 mol/l Na4P2O7，pH 9.1。在用球状蛋白质标准品进行校准后，估算了典型泥炭和煤衍生腐殖酸的表观分子量。借助HEMA柱测定的平均分子量与先前通过在Sephadex柱上进行低压凝胶渗透色谱法获得的值相当。
