检测MAG IgM自身抗体的检测方法的进一步比较。

Further comparisons of assays for detecting MAG IgM autoantibodies.

作者信息

Jaskowski Troy D, Prince Harry E, Greer Ryan W, Litwin Christine M, Hill Harry R

机构信息

Associated Regional and University Pathologists (ARUP), Institute for Clinical and Experimental Pathology, 500 Chipeta Way, Salt Lake City, UT 84108, USA.

出版信息

J Neuroimmunol. 2007 Jul;187(1-2):175-8. doi: 10.1016/j.jneuroim.2007.04.015. Epub 2007 May 29.

DOI:10.1016/j.jneuroim.2007.04.015

PMID:17537521

Abstract

Anti-MAG antibodies are commonly found in the sera of patients with demyelinating sensorimotor neuropathy and IgM paraproteinemia. Our objective here was to compare MAG results obtained by two different laboratories using similar methods (Western blot, EIA, IFA). Western blot (WB) employing MAG from monkey was less sensitive (72.5%) than myelin IFA (92.5%; monkey nerve) and EIA (97.5%; human MAG) when compared to WB using human MAG and is most likely due to methodology (not antigen source). EIA detected low titers of MAG IgM antibodies in suspected patient sera (negative by other methods) that were also SGPG IgM-positive. Patients having low titers by EIA, but negative by WB may have other autoimmune neuropathies without demyelination.

摘要

抗髓鞘相关糖蛋白（MAG）抗体常见于脱髓鞘感觉运动神经病和IgM副蛋白血症患者的血清中。我们在此的目的是比较两个不同实验室使用相似方法（蛋白质印迹法、酶免疫测定法、间接免疫荧光法）获得的MAG检测结果。与使用人MAG的蛋白质印迹法相比，采用猴MAG的蛋白质印迹法（WB）敏感性较低（72.5%），而髓鞘间接免疫荧光法（92.5%；猴神经）和酶免疫测定法（97.5%；人MAG）敏感性更高，这很可能是由于方法学（而非抗原来源）所致。酶免疫测定法在疑似患者血清中检测到低滴度的MAG IgM抗体（其他方法检测为阴性），这些血清同时也是SGPG IgM阳性。酶免疫测定法检测滴度低但蛋白质印迹法检测为阴性的患者可能患有其他无脱髓鞘的自身免疫性神经病。