Ferreira-Castro F L, Aquino S, Greiner R, Ribeiro D H B, Reis T A, Corrêa B
Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, Av. Prof. Lineu Prestes 1374, CEP 05508-900 São Paulo, Brazil.
Appl Radiat Isot. 2007 Aug;65(8):927-33. doi: 10.1016/j.apradiso.2007.03.011. Epub 2007 Mar 28.
The efficacy of gamma-irradiation as a method of decontamination of maize containing Fusarium verticillioides under controlled conditions of relative humidity (RH) (97.5%) and water activity has been studied. Maize grains inoculated with a spore suspension of F. verticillioides were irradiated to 2, 5, and 10 kGy. Thereafter, the irradiated and control samples were analyzed for the presence of fumonisins, their viable cells were counted, and their morphology was investigated by electronic microscopy. It was found possible to decrease the risk of exposure to fumonisins by irradiating maize to 5 or 10 kGy. However, at the dose of 2 kGy, the survived fungi (36%) can produce more fumonisins than the fungi in the control unirradiated samples under the same conditions.
在相对湿度(RH)为97.5%和水分活度的受控条件下,研究了γ射线辐照作为一种对含有轮枝镰孢菌的玉米进行去污处理方法的效果。用轮枝镰孢菌的孢子悬浮液接种的玉米籽粒被辐照至2、5和10千戈瑞。此后,对辐照样品和对照样品进行伏马菌素检测、活菌计数,并通过电子显微镜研究其形态。结果发现,将玉米辐照至5或10千戈瑞可以降低接触伏马菌素的风险。然而,在2千戈瑞的剂量下,存活的真菌(36%)在相同条件下比未辐照的对照样品中的真菌能产生更多的伏马菌素。