直接从患者血清样本中快速检测出赋予更昔洛韦抗性的人巨细胞病毒UL97突变。
Rapid detection directly from patient serum samples of human cytomegalovirus UL97 mutations conferring ganciclovir resistance.
作者信息
Castor Jared, Cook Linda, Corey Lawrence, Jerome Keith R
机构信息
Department of Laboratory Medicine, University of Washington Medical Center, Seattle, Washington 98195, USA.
出版信息
J Clin Microbiol. 2007 Aug;45(8):2681-3. doi: 10.1128/JCM.00526-07. Epub 2007 May 30.
Abstract
Ganciclovir-resistant cytomegalovirus can cause disease and death in transplant recipients. We describe here a rapid PCR- and sequencing-based assay for ganciclovir resistance that can be performed in 1 to 2 working days directly from patient specimens, without the need for amplification of the virus by cell culture. An evaluation of 120 sequential samples submitted for clinical testing revealed a variety of silent and amino acid mutations.
摘要
耐更昔洛韦的巨细胞病毒可导致移植受者发病和死亡。我们在此描述一种基于PCR和测序的快速检测耐更昔洛韦的方法，该方法可直接从患者标本在1至2个工作日内完成，无需通过细胞培养扩增病毒。对提交进行临床检测的120份连续样本的评估揭示了多种沉默突变和氨基酸突变。
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2
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