Park Shin-Young, Lee Sung Hak, Choi Woo Hyuck, Koh Eun Mi, Seo Jee Hee, Ryu Shi Yong, Kim Young Sup, Kwon Dae Young, Koh Woo Suk
Korea Institute of Toxicology, Daejeon, Korea.
Planta Med. 2007 Jun;73(7):674-8. doi: 10.1055/s-2007-981525. Epub 2007 May 31.
Bioactivity-guided fractionation of Saururus chinensis (Saururaceae) using a lymphoproliferation assay led us to isolate 5 lignans (compounds 1 - 5). Compounds 1 - 5 were identified as sauchinone, (-)-saucerneol, saucerneol C, manassantin A, and manassantin B, respectively, by spectroscopic analyses. The immunosuppressive activities of the active compounds were evaluated using lymphoproliferation, mixed leukocyte response, and Th1/Th2 cytokine assays. The relative potency was in the order: manassantin A, B > (-)-saucerneol > saucerneol C > sauchinone.
利用淋巴细胞增殖试验对三白草（三白草科）进行生物活性导向分离，使我们分离出5种木脂素（化合物1 - 5）。通过光谱分析，化合物1 - 5分别被鉴定为柳叶木兰碱、（-）-异日本扁柏酚、异日本扁柏酚C、马萘雌酮A和马萘雌酮B。使用淋巴细胞增殖、混合淋巴细胞反应和Th1/Th2细胞因子测定法评估了活性化合物的免疫抑制活性。相对效力顺序为：马萘雌酮A、B > （-）-异日本扁柏酚 > 异日本扁柏酚C > 柳叶木兰碱。
