Tungtrongchitr Anchalee, Chiworaporn Chutamas, Praewanich Rungson, Radomyos Prayong, Boitano John J
Department of Parasitology, Faculty of Medicine Siriraj Hospital, Mahidol University, 2 Pranok Road, Bangkok Noi, Bangkok 10700, Thailand.
Southeast Asian J Trop Med Public Health. 2007 Mar;38(2):232-8.
A total of 479 stool specimens were collected from rural communities of Ubon Ratchathani Province, Thailand and examined by two techniques: the modified Kato thick smear and the direct smear. The prevalence of Opisthorchis viverrini (14.8%), hookworm (10.2%), Sarcocystis spp (4.6%), Taenia spp (2.9%), Strongyloides stercoralis (2.1%), Giardia lamblia (1.2%), Echinostoma spp (0.6%), Ascaris lumbricoides (0.4%), Entamoeba histolytica (0.2%), Chilomastix mesnili (0.2%) and Endolimax nana (0.2%) were determined. The morphology of the Sarcocystis spp sporocysts examined by both procedures looked similar and was found to be easily recognizable. Among these specimens, 22 cases (4.6%) were positive for Sarcocystis infection detected by the modified Kato technique, whereas only one case (0.2%) was detected by both techniques. These differences were found to be statistically significant (p < 0.05), indicating that the modified Kato technique was decidedly more sensitive than the direct smear procedure in identifying Sarcocystis infection. An epidemiological survey was conducted in Khon Kaen Province involving 1124 stool samples using the modified Kato technique. The greatest frequency was Opisthorchis viverrini at 32.0% while the second highest was Sarcocystis spp at 8.0%. The prevalences of hookworm, Echinostoma spp, Taenia spp, Trichuris trichiura and Enterobius vermicularis were 2.7, 2.1, 1.0, 0.2 and 0.2%, respectively. Other than opisthorchiasis, northeastern Thailand may be an endemic area for sarcocystosis. This is the first report of the applicability and potential usefulness of the Kato thick smear technique for the diagnosis of Sarcocystis infection in a field survey.
从泰国乌汶叻差他尼府的农村社区共采集了479份粪便标本，并采用改良加藤厚涂片法和直接涂片法两种技术进行检测。确定了猫后睾吸虫（14.8%）、钩虫（10.2%）、肉孢子虫属（4.6%）、带绦虫属（2.9%）、粪类圆线虫（2.1%）、蓝氏贾第鞭毛虫（1.2%）、棘口吸虫属（0.6%）、蛔虫（0.4%）、溶组织内阿米巴（0.2%）、梅氏唇鞭毛虫（0.2%）和微小内蜒阿米巴（0.2%）的感染率。通过两种方法检测的肉孢子虫属孢子囊的形态看起来相似，且易于识别。在这些标本中，改良加藤技术检测到22例（4.6%）肉孢子虫感染呈阳性，而两种技术均检测到的仅1例（0.2%）。发现这些差异具有统计学意义（p < 0.05），表明改良加藤技术在识别肉孢子虫感染方面明显比直接涂片法更敏感。在孔敬府使用改良加藤技术对1124份粪便样本进行了流行病学调查。感染率最高的是猫后睾吸虫，为32.0%，其次是肉孢子虫属，为8.0%。钩虫、棘口吸虫属、带绦虫属、鞭虫和蠕形住肠线虫的感染率分别为2.7%、2.1%、1.0%、0.2%和0.2%。除了华支睾吸虫病外，泰国东北部可能是肉孢子虫病的流行地区。这是加藤厚涂片技术在现场调查中诊断肉孢子虫感染的适用性和潜在用途的首次报告。
