Khadka Dhruba Kumar, Eampokalap Boonchuay, Panitchakorn Jarinee, Ramasoota Pongrama, Khusmith Srisin
Department of Microbiology and Immunology, Mahidol University, Bangkok, Thailand.
Southeast Asian J Trop Med Public Health. 2007 Mar;38(2):376-82.
A total of 29 Thai multi-drug-resistant/isoniazid-resistant Mycobacterium tuberculosis isolates were analyzed for mutations in katG from codons 254 to 549, inhA promoter and inhA open reading frame by DNA sequencing and single strand conformation polymorphism. Twenty-five multi-drug resistant isolates exhibited single point mutations (17 isolates at Ser315Thr plus Arg463Leu, 1 at Thr308Pro plus Arg463Leu, 7 at either Ser315Thr or Arg463Leu) while the other 4 isoniazid-resistant isolates had single point mutation only at Arg463Leu. Seven of 25 multi-drug-resistant isolates [4 at C(-15)T, 1 at T(-8)C; 1 at C(-15)T plus Ser94Ala and 1 at Ile21Val] and 2 of 4 isoniazid-resistant isolates [1 at C(-15)T, 1 at C (-15)T plus Ile21Thr] had mutations in inhA promoter and open reading frame, while the other 20 isolates had no mutation at any position. No frame shift mutation was observed in any tested isolates. This is the first report of two mutations, Trp308Pro of katG and T (-8)C of inhA in Mycobacterium tuberculosis isolates.
通过DNA测序和单链构象多态性分析了29株泰国耐多药/耐异烟肼结核分枝杆菌分离株katG基因第254至549密码子、inhA启动子和inhA开放阅读框的突变情况。25株耐多药分离株表现出单点突变（17株为Ser315Thr加Arg463Leu，1株为Thr308Pro加Arg463Leu，7株为Ser315Thr或Arg463Leu之一），而其他4株耐异烟肼分离株仅在Arg463Leu处有单点突变。25株耐多药分离株中的7株[4株为C(-15)T，1株为T(-8)C；1株为C(-15)T加Ser94Ala，1株为Ile21Val]以及4株耐异烟肼分离株中的2株[1株为C(-15)T，1株为C(-15)T加Ile21Thr]在inhA启动子和开放阅读框中有突变，而其他20株分离株在任何位置均无突变。在任何测试分离株中均未观察到移码突变。这是首次报道结核分枝杆菌分离株中katG基因的Trp308Pro和inhA基因的T(-8)C这两种突变。
