Khatsernova B Ia, Silaeva S A, Golenchenko V A, Skuridin S G, Zaĭtsev I I, Rozkin M Ia, Evdokimov Iu M, Rybin V I, Grigorova L V, Efimov V S
Mol Biol (Mosk). 1991 Sep-Oct;25(5):1308-15.
A comparative study of polyconidin effects in the replicative and transcription activity of hepatocyte DNA in intact animals, and on peculiarities of spatial organization DNA in the DNA complex with oligomer-25 conidin was carried out. It was shown that polyconidin binding to DNA results in "cross-linking". This process is accompanied by formation of liquid-crystalline dispersion without abnormal optical activity. Liquid crystals possess high density packing of DNA molecules complexed with quaternary ammonium salt of oligomer-25 conidin. The addition of heparin to liquid-crystalline dispersions or phases, destroys the structure of the DNA complex with quaternary ammonium salt of oligomer-25 conidin. As a result "free" DNA molecules appear, they form the cholesteric liquid-crystalline phase. An increase in transcription activity and synchronization of DNA synthesis in hepatocyte was demonstrated. A correlation between chromatin modification and biological activity of chromatin after formation of the DNA--oligomer-25 conidin complex is proposed.
开展了一项关于多聚康迪菌素对完整动物肝细胞DNA复制和转录活性的影响，以及对与低聚物-25康迪菌素形成的DNA复合物中DNA空间组织特性影响的比较研究。结果表明，多聚康迪菌素与DNA结合会导致“交联”。这一过程伴随着形成无异常光学活性的液晶分散体。液晶具有与低聚物-25康迪菌素季铵盐复合的DNA分子的高密度堆积。向液晶分散体或相中添加肝素会破坏与低聚物-25康迪菌素季铵盐形成的DNA复合物的结构。结果出现了“游离”DNA分子，它们形成胆甾型液晶相。已证明肝细胞中转录活性增加和DNA合成同步。提出了DNA-低聚物-25康迪菌素复合物形成后染色质修饰与染色质生物活性之间的相关性。
