Kostetskiĭ P V, Arkhipova S F, Vladimirova R R
Mol Biol (Mosk). 1991 Sep-Oct;25(5):1345-56.
Homologous amino acid sequences of phospholipases A2 of snakes belonging to families Elapidae, Viperidae and Colubridae were considered in order to study the location of conservative and variable regions. To identify significant conservative and variable regions a comparison between two groups of aligned sequences of snake phospholipases A2 was successfully applied. The phospholipases A2 sequences were divided into two groups (taxons) according to the phylogenetic tree reconstructed from the pair distance matrix. Results of the comparison were plotted to facilitate the identification of significant conservative and variable regions. It was shown, that the results of the comparison between two phylogenetic groups of snake phospholipases A2 didn't depend much on the number of each group representatives, and the location of conservative and variable regions didn't significantly change if one of the groups was represented by the single sequence. It should be mentioned, that the more the phylogenetic difference between groups of phospholipases A2 the more was the number of significant conservative and variable regions. The knowledge of the number and location of conservative and variable regions and their dependence on phylogenetic relations between the compared taxons can be used to predict the synthetic peptide structure to obtain antibodies of various specificity. These antibodies may have either a wide range of cross-reactivity against all of phospholipases A2 or a limited range of cross-reactivity against phospholipases A2 of one taxon.
为了研究保守区和可变区的位置，对眼镜蛇科、蝰蛇科和游蛇科蛇类的磷脂酶A2的同源氨基酸序列进行了分析。为了识别显著的保守区和可变区，成功地对两组比对后的蛇磷脂酶A2序列进行了比较。根据从成对距离矩阵重建的系统发育树，将磷脂酶A2序列分为两组（分类单元）。将比较结果绘制成图，以便于识别显著的保守区和可变区。结果表明，两组蛇磷脂酶A2之间的比较结果在很大程度上不依赖于每组代表的数量，并且如果其中一组由单个序列代表，保守区和可变区的位置也不会有显著变化。应该提到的是，磷脂酶A2组之间的系统发育差异越大，显著保守区和可变区的数量就越多。保守区和可变区的数量和位置及其对所比较分类单元之间系统发育关系的依赖性的知识，可用于预测合成肽结构，以获得具有各种特异性的抗体。这些抗体可能对所有磷脂酶A2具有广泛的交叉反应性，或者对一个分类单元的磷脂酶A2具有有限的交叉反应性。
