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[核小体的结构。使用DNA-蛋白质交联定位与DNA相互作用的H2A和H2B组蛋白片段]

[Structure of nucleosomes. Localization of the H2A and H2B histone segments interacting with DNA using DNA-protein crosslinking].

作者信息

Gushchin D Iu, Ebralidze K K, Mirzabekov A D

出版信息

Mol Biol (Mosk). 1991 Sep-Oct;25(5):1400-11.

PMID:1753964
Abstract

Histones' H2A and H2B peptidic points which interact with nucleosomal DNA have been identified by using the methods of DNA--protein covalent cross-linking. H2B can be linked to DNA via its N-terminal tail and via several lysines contained within residues 24-34. The most prominent site of histone H2A covalent linking to DNA is His-123, the less prominent being Lys-119 and Lys-124.

摘要

通过DNA-蛋白质共价交联方法已鉴定出组蛋白H2A和H2B与核小体DNA相互作用的肽段位点。H2B可通过其N端尾巴以及24-34位残基内所含的几个赖氨酸与DNA相连。组蛋白H2A与DNA共价连接的最显著位点是His-123,较不显著的是Lys-119和Lys-124。

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