Buache Emilie, Garnotel Roselyne, Aubert Dominique, Gillery Philippe, Villena Isabelle
Laboratoire de Biochimie et Biologie Moléculaire, CNRS UMR 6198, IFR 53, UFR Médecine, Université de Reims Champagne-Ardenne, 51 rue Cognacq-Jay, 51095 Reims cedex, France.
Biochem Biophys Res Commun. 2007 Jul 27;359(2):298-303. doi: 10.1016/j.bbrc.2007.05.089. Epub 2007 May 24.
The apicomplexan Toxoplasma gondii, an obligate intracellular parasite, can infect humans and a wide range of vertebrates. Following oral infection, the parasite invades tissues by crossing non-permissive biological barriers such as the placenta or the blood-brain barrier. But the molecular mechanisms underlying migration of T. gondii remain poorly characterized. The crossing of various basal membranes and infiltration into the extracellular matrix by T. gondii could involve matrix metalloproteinases (MMPs). We demonstrated a decrease in proMMP-2 and proMMP-9 secretion by THP-1 cells at 24 and 48h post invasion with regulation at the mRNA level throughout infection. This down regulation was associated with a decrease in TIMP-2 secretion and an inhibition of its expression. Moreover, results showed an activation of MT1-MMP; its expression was regulated after 6, 24, and 48h.
顶复门寄生虫刚地弓形虫是一种专性细胞内寄生虫，可感染人类和多种脊椎动物。经口感染后，该寄生虫通过穿越胎盘或血脑屏障等非允许性生物屏障侵入组织。但刚地弓形虫迁移的分子机制仍不清楚。刚地弓形虫穿越各种基底膜并渗入细胞外基质可能涉及基质金属蛋白酶（MMPs）。我们证明，在感染后24小时和48小时，THP-1细胞分泌的前MMP-2和前MMP-9减少，且在整个感染过程中mRNA水平受到调控。这种下调与TIMP-2分泌减少及其表达抑制有关。此外，结果显示MT1-MMP被激活；其表达在6小时、24小时和48小时后受到调控。
