McKeon T W, Parsons R L
Department of Anatomy and Neurobiology, College of Medicine, University of Vermont, Burlington 05405.
Neuroscience. 1991;45(1):241-54. doi: 10.1016/0306-4522(91)90120-d.
The localization of two cytoskeletal proteins was analysed in the cell bodies and processes of ganglionic neurons and small, intensely fluorescent cells of the parasympathetic cardiac ganglion of Necturus maculosus (mudpuppy). Antibodies against microtubule-associated protein-2 and against the highly phosphorylated isoforms of high and middle molecular weight neurofilament subunits were used as somatodendritic and axonal markers, respectively. The ganglionic neurons, which usually have only one major process, and small, intensely fluorescent cells, which have several processes, showed distinctly different staining patterns with the two antibodies. In control and denervated ganglia, the ganglionic cell bodies and several hundred micrometers of the proximal processes were labeled with the antibody against microtubule-associated protein-2, whereas small, intensely fluorescent cells and processes showed a paucity of immunoreactivity. The neurofilament antibody labeled numerous axons in the ganglion but did not label the proximal part of the postganglionic process or small, intensely fluorescent cell processes. Denervation resulted in the presence of phosphorylated neurofilament subunit immunoreactivity in the soma and proximal process of the ganglionic neuron. These data suggest that (i) small, intensely fluorescent cells and ganglionic neurons in the mudpuppy cardiac ganglion contain distinctly different cytoskeletal proteins, (ii) the proximal part of postganglionic "axons" contains dendrite-like and not axon-like cytoskeletal proteins, and (iii) deafferentation promotes the localization of phosphorylated forms of neurofilament subunits in the soma and proximal process of parasympathetic ganglionic neurons.
对黄斑泥螈（泥狗）交感心脏神经节的神经节神经元以及小而强荧光细胞的胞体和突起中的两种细胞骨架蛋白进行了定位分析。分别使用针对微管相关蛋白2的抗体和针对高分子量和中分子量神经丝亚基的高度磷酸化异构体的抗体作为树突体和轴突标记物。通常只有一个主要突起的神经节神经元和有几个突起的小而强荧光细胞，用这两种抗体显示出明显不同的染色模式。在对照和去神经节中，神经节细胞体和近端几百微米的突起被抗微管相关蛋白2抗体标记，而小而强荧光细胞及其突起的免疫反应性较弱。神经丝抗体标记了神经节中的许多轴突，但未标记节后突起的近端部分或小而强荧光细胞的突起。去神经支配导致神经节神经元的胞体和近端突起中出现磷酸化神经丝亚基免疫反应性。这些数据表明：（i）泥螈心脏神经节中的小而强荧光细胞和神经节神经元含有明显不同的细胞骨架蛋白；（ii）节后“轴突”的近端部分含有树突样而非轴突样的细胞骨架蛋白；（iii）传入神经切断促进了磷酸化形式的神经丝亚基在交感神经节神经元的胞体和近端突起中的定位。
