[Analysis of occurrence of DRB and DQ alleles in sarcoidosis and tuberculosis from Northern Poland].

INTRODUCTION

Sarcoidosis is a multisystem disorder of unknown etiolgy. Pathologic similarities between SA and tuberculosis (TB) suggest M. tuberculosis antigen(s) as causative agents. It seems likely that in the genetically different predisposed hosts, the same antigen(s) may cause the development of sarcoid or tuberculous immune response.

AIM

The aim of this study was to compare the frequency of occurrence of HLA class II alleles in SA, TB and in the healthy individuals.

MATERIAL AND METHODS

To test a difference in haplotypes associated with both diseases, we compared the distribution of DQA1 and DQB1 alleles in 45 SA patients, 62 TB patients and in 143 healthy volunteers, using a PCR-SSP "low (DRB1, DQB1) and high (DQA1) resolution" method.

RESULTS

Our results revealed that DRB103, DRB111, DQB102 i DQA10501 in Stage I of SA with Löfgrens syndrom (Ls) and DRB115, DQA10102, DQA10103 in Stage II of SA were more common, whereas DRB116, DRB104, DRB108, DQB102, DQB103, DQB105, DQA10102, DQA10301 in Ls and DQB102, DQB103, DQB105, DQA10102, DQA10301 in Stage II were less common than in the controls but after Bonferroni correction occurrence of DRB104, DQB102, DQB103, DQB105 and DQA10102, DQA10301, DQA10501 was significantly differ. In TB group, DRB116, DRB114, DQB105 i DQA10303 were more frequent and DRB111, DQB102, DQA10201, DQA10505 less frequently present as compared to the controls, but after correction DRB116, DQB102, DQB105, DQA10303, DQA10505 were significantly different. In SA, DRB111, DQB102 i DQA10201, DQA10501, DQA10505 in Ls and DRB115, DRB111, DQA10102 in Stage II were more common and DRB116, DRB104, DRB114, DQB103, DQB105, DQB106, DQA10301, DQA10302, DQA10303 in Ls and Stage II were less frequent than in the TB group. DQB102, DQA10501 (Ls) and DRB115 (Stage II) were more frequently present in SA than in TB, even after Bonferroni correction.

CONCLUSIONS

In summary, we identified associations of HLA class II alleles in SA and TB with expression pattern specific and different for each group. In most cases, in SA patients frequency of HLA class II alleles occurrence is opposite to the frequency in TB patients.